Improved prime editors enable pathogenic allele correction and cancer modelling in adult mice.
Liu P, Liang SQ, Zheng C, Mintzer E, Zhao YG, Ponnienselvan K, Mir A, Sontheimer EJ, Gao G, Flotte TR, Wolfe SA, Xue W
Nat Commun. 2021 Apr 9;12(1):2121. doi: 10.1038/s41467-021-22295-w. PubMed Article
Liu P, Liang SQ, Zheng C, Mintzer E, Zhao YG, Ponnienselvan K, Mir A, Sontheimer EJ, Gao G, Flotte TR, Wolfe SA, Xue W
Nat Commun. 2021 Apr 9;12(1):2121. doi: 10.1038/s41467-021-22295-w. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 164907 | pAAV-U6-pegSERPINA1-U6-Nicking-PE2-N | Express a pegRNA targeting SERPINA1, and a nicking sgRNA and the N-terminal split-intein fragment of PE2 |
| 164908 | pAAV-PE2-C | Expresses the C-terminal split-intein fragment of PE2 |
| 164909 | pAAV-PE2-N | Expresses the N-terminal split-intein fragment of PE2 |
| 169841 | pegAAT K342E correction | Express a pegRNA used for correction (via A•T-to-G•C) of the E342K mutation |
| 169842 | sgAAT Nicking | Express a nicking sgRNA used for correction (via A•T-to-G•C) of the E342K mutation |
| 169843 | pegCTNNB1 S45F | pegRNA used for installation (via C•G-to-T•A) of the oncogenic S45F in Ctnnb1 in mouse liver. |
| 169844 | pegCTNNB1 S45del | pegRNA used for installation (via TCC deletion) of the oncogenic S45del in Ctnnb1 in mouse liver. |
| 169845 | sgCTNNB1 Nicking | Express a nicking sgRNA used for installation of the oncogenic S45F or S45del in Ctnnb1 in mouse liver. |
| 169850 | SpyCas9 PE2* | Mammalian Expression, SpyCas9 prime editor, optimized NLS |
| 169851 | SauCas9 PE2* | Mammalian Expression, sauCas9 based prime editor |
| 169852 | SauKKHCas9 PE2* | Mammalian Expression, sauCas9-KKH based prime editor |
| 169853 | SpyCas9 PE2-3HA | Mammalian Expression, SpyCas9 prime editor with 3HA-tag |
| 169854 | SpyCas9 PE2*-3HA | Mammalian Expression, SpyCas9 PE2* prime editor with 3HA-tag |
| 169855 | Spy EMX1-pegRNA | SpyCas9-pegRNA for EMX1 |
| 169856 | Spy CCR5-pegRNA | SpyCas9-pegRNA for CCR5 |
| 169857 | SauCas9 EMX1-pegRNA | SauCas9 pegRNA for EMX1 |
| 169859 | SpyCas9 EMX1-nicking sgRNA | SpyCas9 nicking sgRNA for EMX1 |
| 169860 | SauCas9 EMX1-nicking sgRNA | SauCas9 nicking sgRNA for EMX1 |
| 169863 | SauCas9KKH CCR5-nicking sgRNA | SauCas9KKH nicking sgRNA for CCR5 |