sgCTNNB1 Nicking
(Plasmid
#169845)
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PurposeExpress a nicking sgRNA used for installation of the oncogenic S45F or S45del in Ctnnb1 in mouse liver.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 169845 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepmd127
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesgCTNNB1 Nicking
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gRNA/shRNA sequenceGAAAAGCTGCTGTCAGCCAC
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SpeciesM. musculus (mouse)
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GenBank ID
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Entrez GeneCtnnb1 (a.k.a. Bfc, Catnb, Mesc)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer HU6F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
sgCTNNB1 Nicking was a gift from Wen Xue (Addgene plasmid # 169845 ; http://n2t.net/addgene:169845 ; RRID:Addgene_169845) -
For your References section:
Improved prime editors enable pathogenic allele correction and cancer modelling in adult mice. Liu P, Liang S, Zheng C, Mintzer E, Zhao YG, Ponnienselvan K, Mir A, Sontheimer EJ, Gao G, Flotte TR, Wolfe SA, Xue W. BioRxiv 10.1101/2020.12.15.422970