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(Plasmid #168122)


Item Catalog # Description Quantity Price (USD)
Plasmid 168122 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    Clontech-style N1
  • Backbone manufacturer
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    GNAI2, GNB1, GNG2
  • Species
    H. sapiens (human)
  • Mutation
    NLuc is inserted at A114/E115 within GNAI1
  • Entrez Gene
    GNAI1 (a.k.a. Gi, HG1B, NEDHISB)
  • Entrez Gene
    GNB1 (a.k.a. HG2A, MDS, MRD42)
  • Entrez Gene
    GNG2 (a.k.a. HG3F1)
  • Tag / Fusion Protein
    • cpVenus on GNG2 (N terminal on insert)

Cloning Information

  • Cloning method Unknown

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Gi3-CASE was a gift from Gunnar Schulte (Addgene plasmid # 168122 ; ; RRID:Addgene_168122)
  • For your References section:

    Quantitative assessment of constitutive G protein-coupled receptor activity with BRET-based G protein biosensors. Schihada H, Shekhani R, Schulte G. Sci Signal. 2021 Sep 7;14(699):eabf1653. doi: 10.1126/scisignal.abf1653. Epub 2021 Sep 7. 10.1126/scisignal.abf1653 PubMed 34516756