163,404 results

1. pg-HIF-1alpha-EGFP

   Plasmid

   #87204

   Purpose

   C-terminally EGFP-tagged human HIF-1a

   Depositor

   Violaine Sée

   Article

   Bagnall et al J Biol Chem. 2014 Feb 28;289(9):5549-64.

   Insert

   HIF-1a (HIF1A Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   L40F, M88V, Q814R

   Promoter

   CMV

   Available since

   Sept. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-nanoHER2-SpyTag003

   Plasmid

   #216312

   Purpose

   Expresses nanoHER2-SpyTag003 in bacterial cells. nanoHER2 is an anti-HER2 nanobody.

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   nanoHER2-SpyTag003
   

   Use

   Tags

   His6 tag and SpyTag003

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pICH41744

   Plasmid

   #48017

   Depositor

   Sylvestre Marillonnet

   Article

   Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

   Type

   Empty backbone

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 3, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pmTurquoise2-Peroxi

   Plasmid

   #36203

   Purpose

   In vivo visualization of the peroxisome (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

   Insert

   Peroxisomal targeting sequence
   

   Use

   Tags

   mTurquoise2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAG413GPD-ccdB

   Plasmid

   #14142

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMXs-mGCN2-FLAG-IP

   Plasmid

   #101794

   Purpose

   Retroviral vector encoding C-terminally FLAG tagged mouse GCN2

   Depositor

   Seiichi Oyadomari

   Article

   Taniuchi et al Sci Rep. 2016 Sep 16;6:32886. doi: 10.10

   Insert

   Gcn2 (Eif2ak4 Mouse)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-N1-GRASP55

   Plasmid

   #137708

   Purpose

   Eukaryotic expression of rat GRASP55 full-length

   Depositor

   Yanzhuang Wang

   Insert

   GRASP55
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPAGFP-VSVG

   Plasmid

   #11915

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Presley et al Nature. 1997 Sep 4. 389(6646):81-5.

   Insert

   VSVG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   (L64F/T65S/V163A/T203H). The PAGFP also contains …

   Promoter

   Available since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q145

   Plasmid

   #111731

   Purpose

   Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

   Depositor

   Cheryl Arrowsmith

   Article

   Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

   Insert

   HTT (HTT Human)
   

   Use

   Baculovirus expression

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Q145 (polyQ repeat)

   Promoter

   CMV and P10

   Available since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLX302

    Plasmid

    #25896

    Depositor

    David Root

    Article

    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pETDuet-1 TIM9,10

    Plasmid

    #170280

    Purpose

    Expresses both yeast Tim9 and yeast Tim10 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production

    Depositor

    Paul Schanda

    Article

    Weinhaupl et al Cell. 2018 Nov 15;175(5):1365-1379.e25.

    Insert

    mitochondrial Tim9 chaperone subunit and Tim10 (with N-ter His tag) (TIM9, TIM10 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tim9: via NdeI/XhoI into the MCS2; Tim10: via Bam…

    Available since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pToneDest

    Plasmid

    #67691

    Purpose

    attR4-attR3 Destination cassette in Tol1 backbone

    Depositor

    Nathan Lawson

    Article

    Shin et al Development. 2016 Oct 15;143(20):3785-37

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDONR223-EGFR

    Plasmid

    #23935

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    EGFR (EGFR Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. psiCHECK2-Eef2TOP-RLCP-HCVIRES-FL

    Plasmid

    #233628

    Purpose

    Expression of bicistronic reporter with TOP motif

    Depositor

    Shintaro Iwasaki

    Article

    Shichino et al Nat Struct Mol Biol. 2024 Oct;31(10):155

    Insert

    Promoter and 5'UTR of Eef2 gene and HCV IRES (Eef2 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-tMCK-ER-TurboID

    Plasmid

    #149412

    Purpose

    Muscle-specific expression of ER-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

    Depositor

    Jonathan Long

    Article

    Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

    Insert

    ER-TurboID
    

    Use

    AAV

    Tags

    HA and V5

    Expression

    Mutation

    Promoter

    tMCK

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENTR4-HaloTag (w876-1)

    Plasmid

    #29644

    Depositor

    Eric Campeau

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    HaloTag

    Expression

    Mutation

    Promoter

    Available since

    May 11, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. AiP13572 - pAAV-AiE0390h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3572)

    Plasmid

    #208143

    Purpose

    AiE0390h_3xC2 is an optimized enhancer sequence designed to drive AAV-mediated transgene expression in brain astrocytes

    Depositor

    The Allen Institute for Brain Science, Jonathan Ting

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    Beta Globin minimal promoter

    Available since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. CIBN-CAAX

    Plasmid

    #79574

    Purpose

    expression of N-terminal portion of CIB1 with a C-terminal CAAX box from KRras for plasma-membrane targeting

    Depositor

    Olof Idevall-Hagren, Pietro De Camilli

    Article

    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

    Insert

    CIBN (CIB1 Mustard Weed)
    

    Use

    Tags

    C-terminal CAAX-box

    Expression

    Mammalian

    Mutation

    K93A, R94A, K106A, K107A (please see depositor co…

    Promoter

    CMV

    Available since

    July 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. 35S-GWY-VP16/pAM

    Plasmid

    #201232

    Purpose

    Used to produce and express in planta C-terminal fusion proteins with the VP16 activation domain

    Depositor

    Michael Ayliffe

    Article

    Chen et al Plant J. 2023 Jun;114(6):1209-1226. doi:

    Insert

    35S-GWY-VP16
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. EF1a- gfp-2a-puro

    Plasmid

    #129443

    Purpose

    expression of GFP and Puro R

    Depositor

    Oskar Laur

    Insert

    gfp-puro
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge