Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pAAV-GFAP104-melanopsin-mCherry
(Plasmid #122630)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 122630 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAAV-GFAP104
  • Backbone manufacturer
    stratagene
  • Backbone size w/o insert (bp) 4906
  • Total vector size (bp) 7093
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Can use DH5alpha at 37°C or Stbl3 at 30°C. Carbenicillin is preferred over ampicillin. In DH5alpha this plasmid may act more like a high copy plasmid, although in Stbl3 it may act more like a low copy plasmid.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    melanopsin-mCherry
  • Species
    Synthetic
  • Insert Size (bp)
    2187
  • GenBank ID
  • Promoter GFAP104
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer ggagagctctccccatag
  • 3′ sequencing primer cagcgtatccacatagcg
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The plasmid is fully sequenced in the coding sequence regions (opsin-fluorophore and important flanking regions). Multiple digestions were done to verify the vector structure. The construct and the virus were both tested in vitro.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-GFAP104-melanopsin-mCherry was a gift from Edward Boyden (Addgene plasmid # 122630 ; http://n2t.net/addgene:122630 ; RRID:Addgene_122630)
  • For your References section:

    Melanopsin for precise optogenetic activation of astrocyte-neuron networks. Mederos S, Hernandez-Vivanco A, Ramirez-Franco J, Martin-Fernandez M, Navarrete M, Yang A, Boyden ES, Perea G. Glia. 2019 May;67(5):915-934. doi: 10.1002/glia.23580. Epub 2019 Jan 11. 10.1002/glia.23580 PubMed 30632636