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PurposeEngineered PcrA_M6 helicase to support isothermal amplification method SHARP
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 194156 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET-11b
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Backbone manufacturerN/A
- Backbone size w/o insert (bp) 5676
- Total vector size (bp) 7971
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsProtein expression in BL21 (DE3) pLysS
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEngineered helicase PcrA_M6
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SpeciesN/A
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Insert Size (bp)2295
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MutationH93A, C96A, N187C, C247A, L384V, L409C
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Tags
/ Fusion Proteins
- 6x His tag (N terminal on insert)
- FLAG tag (C terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET-11b_PcrA_M6_H93A was a gift from Taekjip Ha (Addgene plasmid # 194156 ; http://n2t.net/addgene:194156 ; RRID:Addgene_194156) -
For your References section:
Engineered helicase replaces thermocycler in DNA amplification while retaining desired PCR characteristics. Gavrilov M, Yang JYC, Zou RS, Ma W, Lee CY, Mohapatra S, Kang J, Liao TW, Myong S, Ha T. Nat Commun. 2022 Oct 23;13(1):6312. doi: 10.1038/s41467-022-34076-0. 10.1038/s41467-022-34076-0 PubMed 36274095
