pET22B_SA_PcrA
(Plasmid #102999)

• Purpose: The plasmid is used to express Staphylococcus aureus PcrA, a DNA helicase, with N-terminal His-tag and Gly-Gly linker
• Depositing Lab: Martin Webb
• Publication: Webb Lab plasmids (unpublished)

Backbone

• Vector backbone: pET22B
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Growth instructions: B834 (DE3) pLysS for protein production
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SA_PcrA
• Species: Staphylococcus aureus
• Mutation: PcrA 199-2423
• Promoter: T7
• Tag / Fusion Protein:
  • His tag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: T7

Resource Information

• Supplemental Documents:
  • pET22B_SA_PcrA information and protocol
• A portion of this plasmid was derived from a plasmid made by: Synthetically produced by GenScript (The Biology CRO, U.S.A.)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry