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pET22B_SA_PcrA
(Plasmid #102999)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 102999 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    B834 (DE3) pLysS for protein production
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SA_PcrA
  • Species
    Staphylococcus aureus
  • Mutation
    PcrA 199-2423
  • Promoter T7
  • Tag / Fusion Protein
    • His tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET22B_SA_PcrA was a gift from Martin Webb (Addgene plasmid # 102999 ; http://n2t.net/addgene:102999 ; RRID:Addgene_102999)