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Donna Webb Lab: Webb Lab plasmids
Unpublished

Plasmids from Article

ID Plasmid Purpose  
102783APPL1-N308D-GFPPoint mutation in APPL1 that diminishes its interaction with Rab5
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102784APPL1-N308D-mCherryPoint mutation in APPL1 that diminishes its interaction with Rab5
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102785Rab5-L38R-mCherryPoint mutation in Rab5 that reestablishes interaction with APPL1 when co-expressed with APPL1-N308D
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102998pET22B_PcrA_2BchimeraExpress a DNA helicase, a chimeric construct of Bacilllus stearothermophilus PcrA and 2B domain of Staphylococcus aureus PcrA
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102999pET22B_SA_PcrAThe plasmid is used to express Staphylococcus aureus PcrA, a DNA helicase, with N-terminal His-tag and Gly-Gly linker
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