pGV3341
(Plasmid
#170279)
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PurposeExpression of mutant HIV-2 RT p66 reverse transcriptase
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 170279 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCDFDuet-1
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 3498
- Total vector size (bp) 5091
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Streptomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameHIV-2 RT p66 (mutant)
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Alt nameHIV-2 reverse transcriptase, full length (mutant)
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SpeciesHIV-2
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Insert Size (bp)1683
- Promoter T7-lac
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.medrxiv.org/content/10.1101/2020.08.13.20173757v4 for medRxiv preprint
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGV3341 was a gift from Gregory Van Duyne (Addgene plasmid # 170279 ; http://n2t.net/addgene:170279 ; RRID:Addgene_170279) -
For your References section:
Detection of SARS-CoV-2 RNA using RT-LAMP and molecular beacons. Sherrill-Mix S, Hwang Y, Roche AM, Glascock A, Weiss SR, Li Y, Haddad L, Deraska P, Monahan C, Kromer A, Graham-Wooten J, Taylor LJ, Abella BS, Ganguly A, Collman RG, Van Duyne GD, Bushman FD. Genome Biol. 2021 Jun 3;22(1):169. doi: 10.1186/s13059-021-02387-y. 10.1186/s13059-021-02387-y PubMed 34082799
