Engineered helicase replaces thermocycler in DNA amplification while retaining desired PCR characteristics.
Gavrilov M, Yang JYC, Zou RS, Ma W, Lee CY, Mohapatra S, Kang J, Liao TW, Myong S, Ha T
Nat Commun. 2022 Oct 23;13(1):6312. doi: 10.1038/s41467-022-34076-0. PubMed Article
Gavrilov M, Yang JYC, Zou RS, Ma W, Lee CY, Mohapatra S, Kang J, Liao TW, Myong S, Ha T
Nat Commun. 2022 Oct 23;13(1):6312. doi: 10.1038/s41467-022-34076-0. PubMed Article
Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
194156 | pET-11b_PcrA_M6_H93A | Engineered PcrA_M6 helicase to support isothermal amplification method SHARP |