161,273 results

1. Strep-His-Tev-hCGN

   Plasmid

   #236512

   Purpose

   For expression of Cingulin (human) in insect cells

   Depositor

   Sandra Citi

   Article

   Rouaud et al J Cell Biol. 2023 Jul 3;222(7):e20220806

   Insert

   CGN (CGN Human)
   

   Use

   Tags

   2xStrep-10xHis-Tev

   Expression

   Insect

   Mutation

   WT

   Promoter

   Available since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. AiP15285 - pAAV-AiE0170h_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN5285)

   Plasmid

   #230509

   Purpose

   AiE0170h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

   Depositor

   Jonathan Ting, The Allen Institute for Brain Science

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   iCre(R297T)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   minBG

   Available since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV.Ple155-CI-mRuby2-W3SL_BbsI(GGA)

   Plasmid

   #231364

   Purpose

   Ple155-driven mRuby2, containing cassette for BbsI-based Golden Gate assembly (e.g. of sgRNA cassette(s)). Can be used as 'no guide' control.

   Depositor

   Viviana Gradinaru

   Article

   Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

   Insert

   mRuby2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAIP1

   Plasmid

   #233685

   Purpose

   Engineered SUMO protease expression plasmid designed for efficient protease-mediated elution.

   Depositor

   Christopher Bahl

   Insert

   Ulp1-like SUMO protease
   

   Use

   Tags

   CAHS-107838 and HisTag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 4, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. SJZ5-HPH

   Plasmid

   #228258

   Purpose

   Template plasmid for yeast gene knockout, confers antibiotic resistance to hygromycin B

   Depositor

   Zhiping Xie

   Article

   Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

   Insert

   hph
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SSA1 S.c.

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR/zeo-MACROD1

   Plasmid

   #172572

   Purpose

   For gateway cloning of N-terminal tagged MACROD1 constructs.

   Depositor

   Karla Feijs

   Article

   Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

   Insert

   MACROD1 (MACROD1 Human)
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV_RTN3E-mScarlet3

   Plasmid

   #216255

   Purpose

   mammalian expression vector for RTN3E-mScarlet3

   Depositor

   Edward Avezov

   Article

   Crapart et al Proc Natl Acad Sci U S A. 2024 Mar 26;12

   Insert

   reticulon 3 isoform e (RTN3 Human)
   

   Use

   Tags

   mScarlet3

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMT-C34-H

   Plasmid

   #223966

   Purpose

   Anti-MT-C34 heavy chain expression plasmid; to be used with the light chain plasmid, pMT-C34-L (Plasmid 223967), to make the antibody.

   Depositor

   Dmitriy Mazurov

   Article

   Kalinichenko et al Biology (Basel). 2024 Aug 29;13(9):675.

   Insert

   Variable domain of mouse anti-MT-C34 IgG1 heavy chain jointed to constant region of human IgG1 heavy chain
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPOTv7-blast-mStayGold[E138D]

   Plasmid

   #221049

   Purpose

   Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

   Depositor

   Samuel Dean

   Article

   Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

   Insert

   mStayGold[E138D]
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   read through

   Available since

   Aug. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC19-HDRT-TRAC-CD19.CAR-Cas12a.PAM.mutated

    Plasmid

    #215769

    Purpose

    encodes for HDR template for optimized AsCas12a-mediated knock-in of a CD19-specific CAR into the TRAC locus

    Depositor

    Dimitrios Wagner

    Article

    Glaser et al Genome Biol. 2023 Apr 24;24(1):89. doi:

    Inserts

    TRAC-5' homology arm (TRAC Human)
    P2A-CD19.28.zeta-bgH (CD19 Bovine, Mouse, Human)
    TRAC-3' homology arm (TRAC Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Cas12a PAM mutated

    Promoter

    Available since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. AA304

    Plasmid

    #216072

    Purpose

    Fragmid fragment: (guide cassette) guide expression

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; BsmBI_v0; BsmBI_v1; trRNA_v1 [Sa]; terminator_v1
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUPD2-HR1bR181A R185A-C (B3-B4)

    Plasmid

    #211855

    Purpose

    CDS for mutant HR1b degron in domestication vector

    Depositor

    Marcela Rojas-Pierce

    Article

    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

    Insert

    HR1b
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    R181A R185A

    Promoter

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUPD2-3xNLS-HA-LRR-NEL-C (B3-B4)

    Plasmid

    #211861

    Purpose

    Nuclear-localized E3-DART ligase in domestication vector.

    Depositor

    Marcela Rojas-Pierce

    Article

    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

    Insert

    LRR-NEL
    

    Use

    Synthetic Biology

    Tags

    3xNLS-HA

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. TFORF0406

    Plasmid

    #143023

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    CTCFL (CTCFL Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pD649-HAsp-PTGFRN-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156982

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    PTGFRN (PTGFRN Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR/zeo-MACROD2

    Plasmid

    #172573

    Purpose

    For gateway cloning of N-terminal tagged MACROD2 constructs.

    Depositor

    Karla Feijs

    Article

    Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

    Insert

    MACROD2 (MACROD2 Human)
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hSyn-mCherry-WPRE

    Plasmid

    #127860

    Purpose

    pAAV plasmid expressing mCherry under the hSyn promoter

    Depositor

    Florian Freudenberg

    Article

    Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

    Insert

    mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-EGFP-Cdc42(wt)

    Plasmid

    #12599

    Depositor

    Klaus Hahn

    Article

    Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

    Insert

    EGFP-Cdc42 (WT) (CDC42 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHR-OptoCAR-mScarlet

    Plasmid

    #170463

    Purpose

    Expresses an optically-controllable chimeric antigen receptor that is dependent on dimerizer drug, fused to mScarlet fluorophore

    Depositor

    John James

    Article

    Harris et al Mol Syst Biol. 2021 May;17(5):e10091. do

    Insert

    OptoCAR
    

    Use

    Lentiviral

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRS305-Leu2-PINO4-PfV-GS-Sapphire

    Plasmid

    #116930

    Purpose

    Yeast integrative vector for the exression of the 40nm GEM under the INO4 promoter

    Depositor

    Liam Holt

    Article

    Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

    Insert

    Pfv
    

    Use

    Tags

    Sapphire

    Expression

    Yeast

    Mutation

    Promoter

    INO4

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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