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pAAV-S5E2-FingR-PSD95-mGL

Plasmid

#217533

Purpose

Genetically encoded fluorescent probe for labelling endogenous excitatory synapses on PV interneurons in vivo

Depositor

Peter Scheiffele

Article

Okur et al bioRxiv 2023

Insert

ZnF-FingR-PSD95-mGreenLantern (Dlg4 Mouse)

Use

AAV

Tags

mGreenLantern

Expression

Mammalian

Mutation

Promoter

S5E2 enhancer

Available since

April 11, 2024

Availability

Academic Institutions and Nonprofits only

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TRPC1-FLAG

Plasmid

#24408

Depositor

Craig Montell

Article

Xu et al Cell. 1997 Jun 27. 89(7):1155-64.

Insert

TRPC1 (TRPC1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-SARS-CoV-2-nsp7-2xStrep-IRES-Puro

Plasmid

#141373

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp7 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available since

April 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLEX307 RFP

Plasmid

#163976

Purpose

Mammalian expression vector encoding RFP (for example, to normalize plasmid-based protein expression)

Depositor

Daniel Bachovchin

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

RFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pGW1-Myc-DJ1-WT

Plasmid

#29347

Depositor

Mark Cookson

Article

Miller et al J Biol Chem. 2003 Sep 19. 278(38):36588-

Insert

DJ1 (PARK7 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

April 1, 2011

Availability

Academic Institutions and Nonprofits only

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pRRL MND WASp

Plasmid

#36248

Depositor

David Rawlings

Article

Astrakhan et al Blood. 2012 Mar 19.

Insert

WASp (WAS Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

MND

Available since

May 15, 2012

Availability

Academic Institutions and Nonprofits only

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pLX302_FOXA1-V5

Plasmid

#70090

Purpose

Expression of human FOXA1 with C-terminal V5 tag, puromycin selection marker, 3rd generation lentiviral vector

Depositor

William Hahn

Article

Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

Insert

FOXA1 (FOXA1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pBabe bleo human PPAR gamma 2

Plasmid

#11439

Depositor

Ronald Kahn

Insert

PPAR gamma 2 (PPARG Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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pLIX-hN1ICD

Plasmid

#91897

Purpose

Doxycycline inducible expression of human Notch1 ICD

Depositor

Julien Sage

Insert

Notch1 intracellular domain (NOTCH1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

codons 1770 to 2555 of human NOTCH1

Promoter

TRE promoter, Tet ON

Available since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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pMJ806

Plasmid

#39312

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 7, 2012

Availability

Academic Institutions and Nonprofits only

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pEB-C5

Plasmid

#28213

Purpose

5 reprogramming factors (OCT4, SOX2, KLF4, c-MYC and LIN28) are expressed as a single poly-cistronic unit

Depositor

Linzhao Cheng

Article

Chou et al Cell Res. 2011 Jan 18. ():.

Insert

Oct4, Sox2, Klf4, c-Myc, Lin28 (Myc, Sox2, Pou5f1, Klf4, Lin28a Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mhYFP

Plasmid

#186533

Purpose

Monomeric hyperfolder YFP fluorescent protein: cytosolic expression in mammalian cells

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Monomeric hyperfolder YFP

Use

Tags

Expression

Mammalian

Mutation

hfYFP-S147P/L195M/V206K

Promoter

CMV

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pSLIK-IDH1-R132H-FLAG

Plasmid

#66803

Purpose

Tet-inducible expression of mutant IDH1 in mammalian cells, 3rd generation lentiviral vector

Depositor

Christian Metallo

Article

Lewis et al Mol Cell. 2014 Jul 17;55(2):253-63. doi:

Insert

IDH1 (IDH1 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

R132H

Promoter

CMV

Available since

Feb. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pAT15516_BEAR-GFP-2in1

Plasmid

#174082

Purpose

BEAR-GFP plasmid with a gRNA that targets its own plasmid

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

EGFP split with an intron between amino acids 95-96

Use

CRISPR

Tags

Expression

Mammalian

Mutation

disrupted 5' splice site

Promoter

CMV

Available since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-RhoA-T19N

Plasmid

#12963

Depositor

Gary Bokoch

Insert

RhoA DN (RHOA Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

T19N Dominant negative

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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hSOD1-FLAG

Plasmid

#182922

Purpose

Mammalian expression plasmid for human WT SOD1

Depositor

Philip Eaton

Article

Switzer et al Proc Natl Acad Sci U S A. 2023 Jan 17;12

Insert

[Cu-Zn] Superoxide dismutase 1 (SOD1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pLIB-HIS-TEV-scCAK1

Plasmid

#177014

Purpose

Generate baculovirus for insect cell expression of scCAK1 with N-terminal Polyhistidine-TEV

Depositor

Andrea Musacchio

Article

Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

Insert

CAK1 cyclin-dependent protein kinase-activating kinase CAK1 (CAK1 Budding Yeast)

Use

Tags

Polyhistidine-TEV

Expression

Insect

Mutation

Promoter

Available since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-HcKCR1-EYFP

Plasmid

#182021

Purpose

Express light-gated potassium channel HcKCR1-EYFP in mammalian cells

Depositor

Mingshan Xue

Article

Govorunova et al Nat Neurosci. 2022 Jun 20. pii: 10.1038/

Insert

HcKCR1

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Available since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti PGK GFP Blast (w510-5)

Plasmid

#19069

Purpose

3rd gen lentiviral eGFP expression vector, PGK promoter, Blast

Depositor

Paul Kaufman, Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

enhanced green fluorescent protein

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pHAGE-EGFR-T790M

Plasmid

#116310

Purpose

Lentiviral expression of EGFR T790M

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EGFR (EGFR Human)

Use

Lentiviral

Tags

Expression

Mutation

T790M

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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