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pHMT.wt (p53)
(Plasmid #12139)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 12139 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pM2.6
  • Backbone manufacturer
    M.J. Sleigh (CSIRO)
  • Backbone size w/o insert (bp) 4630
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    p53
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1500
  • Entrez Gene
    Trp53 (a.k.a. Tp53, bbl, bfy, bhy, p44, p53)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer na
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Murine wild-type p53 cDNA was excised from plasmid
pLCRcala (Sherley, 1991) by digestion with BamHI and TfiI. The restriction fragment ends were filled in with Klenow and ligated to HindIII linkers.
Following digestion with HindIII, the p53 fragment was inserted into the HindIII site of the
plasmid pM2.6, downstream of the Zn-responsive
human metallothionein promoter modified to provide
low levels of basal gene expression (McNeall et al.,
1989).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHMT.wt (p53) was a gift from James Sherley (Addgene plasmid # 12139 ; http://n2t.net/addgene:12139 ; RRID:Addgene_12139)
  • For your References section:

    Comparison of bax, waf1, and IMP dehydrogenase regulation in response to wild-type p53 expression under normal growth conditions. Liu Y, Riley LB, Bohn SA, Boice JA, Stadler PB, Sherley JL. J Cell Physiol. 1998 Nov . 177(2):364-76. 10.1002/(SICI)1097-4652(199811)177:2<364::AID-JCP18>3.0.CO;2-9 PubMed 9766533