pBabe Nf2 wild-type
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14116||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5169
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Entrez GeneNf2 (a.k.a. merlin)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pBABE 5' (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBabe Nf2 wild-type was a gift from Tyler Jacks (Addgene plasmid # 14116 ; http://n2t.net/addgene:14116 ; RRID:Addgene_14116)
For your References section:Cellular transformation by a FERM domain mutant of the Nf2 tumor suppressor gene. Johnson KC, Kissil JL, Fry JL, Jacks T. Oncogene. 2002 Sep 5. 21(39):5990-7. 10.1038/sj.onc.1205693 PubMed 12203111