|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11342||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturersee "author's map". Not the same as Clontech's pLexA.
- Backbone size (bp) 5700
Vector typeYeast Expression
Growth in Bacteria
Copy numberHigh Copy
/ Fusion Protein
- LexA DNA Binding Domain (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer LexA sequencing primer (CGTCAGCAGAGCTTCACCATTG) (Common Sequencing Primers)
Yeast two-hybrid DNA-binding domain vector, containing the MCS illustrated in the author's map. Use for traditional cloning for a target cDNA sequence for a yeast two-hybrid screen.
This plasmid is intended for use exclusively as a teaching resource as part of the Integrated Genomics Discovery-Based Laboratory Course. Addgene does not make any guarantee that the plasmid is suitable for research purposes.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLexA was a gift from Guy Caldwell (Addgene plasmid # 11342 ; http://n2t.net/addgene:11342 ; RRID:Addgene_11342)
Map uploaded by the depositor.