We narrowed to 19,677 results for: Cre

1. TA1-DuET

   Plasmid

   #213382

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   TA1 (TAAR1 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiCRISPR-v1-sgGLS_2

   Plasmid

   #163459

   Purpose

   lentiviral vector expressing Cas9 and an sgRNA targeting GLS

   Depositor

   Jason Cantor

   Article

   Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

   Insert

   sgRNA 2 targeting GLS (GLS Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5FRT/TO-Cezanne-Strep-HA

   Plasmid

   #113492

   Purpose

   Expression of human Cezanne with C-terminal strep-HA tag

   Depositor

   Hemmo Meyer

   Article

   Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

   Insert

   Cezanne (OTUD7B Human)
   

   Tags

   2xstrep-HA

   Expression

   Mammalian

   Mutation

   wildtype without stop codon

   Promoter

   CMV

   Available Since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLentiRNACRISPR_002 - hU6-DR_BsmBI-EFS-PguCas13b-NES-2A-Puro-WPRE

   Plasmid

   #138144

   Purpose

   Expresses PguCas13b in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang cacc. R overhang caac

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   PguCas13b
   

   Use

   Lentiviral

   Tags

   NES

   Available Since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. Plxna2-AP-His

   Plasmid

   #71997

   Purpose

   Expresses the extracellular region of the PlexinA2 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Plxna2 (Plxna2 Mouse)
   

   Tags

   AP-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLCHKOv3

   Plasmid

   #209025

   Purpose

   Lentiviral backbone for expressing U6 driven hybrid guide (hg)RNAs with BveI cloning sites and puromycin selection marker

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. 783 pCScherryActMEK

   Plasmid

   #31880

   Depositor

   Nathan Lawson

   Article

   Covassin et al Dev Biol. 2009 May 15. 329(2):212-26.

   Insert

   MEK1 (MAP2K1 Human)
   

   Use

   Expression vector

   Tags

   mCherry

   Mutation

   Ser218Glu Ser222Asp; activated MEKK

   Promoter

   CMV

   Available Since

   Sept. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

8. H2B-mAvicFP1

   Plasmid

   #129511

   Purpose

   Expresses H2B-mAvicFP1 in mammalian cells (human histone 2B)

   Depositor

   Nathan Shaner

   Article

   Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

   Insert

   H2B-mAvicFP1
   

   Expression

   Mammalian

   Mutation

   mAvicFP1 fused to the C terminus of human histone…

   Promoter

   CMV

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV_RBMX-eA3A-UdgX-nCas9

   Plasmid

   #163546

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   RBMX-eA3A-UdgX-nCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)

   Available Since

   Dec. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. RXFP1-DuET

    Plasmid

    #213373

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    RXFP1 (RXFP1 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pECE-M2-PPP1R12C wt

    Plasmid

    #31659

    Depositor

    Anne Brunet

    Article

    Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

    Insert

    Protein Phosphatase 1 Regulatory Subunit 12C (PPP1R12C Human)
    

    Tags

    M2

    Expression

    Mammalian

    Mutation

    codon-optimized

    Available Since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pCDH_TNF-SBP-mCherry

    Plasmid

    #65283

    Purpose

    TNF reporter only (no hook) (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    TNF (TNF Human)
    

    Use

    Lentiviral

    Tags

    Streptavidin Binding Protein (SBP) and mCherry

    Promoter

    CMV

    Available Since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLJC6-METAP1-3xFLAG

    Plasmid

    #163453

    Purpose

    lentiviral overexpression vector for human METAP1

    Depositor

    Jason Cantor

    Article

    Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

    Insert

    METAP1 (METAP1 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    See: comments

    Promoter

    Ubc

    Available Since

    March 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. Nrp2.3-AP-His

    Plasmid

    #71974

    Purpose

    Expresses the extracellular region of the Neuropilin 2, isoform 3 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Nrp2.3 (Nrp2 Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNCST-mAvicFP1

    Plasmid

    #129509

    Purpose

    Expresses mAvicFP1 constitutively in E. coli (most strains)

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    mAvicFP1
    

    Expression

    Bacterial

    Promoter

    synthetic constitutive (stationary phase) promote…

    Available Since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. Ncam1_1.18.1-Fc-His

    Plasmid

    #72088

    Purpose

    Expresses the extracellular region of the NCAM1, isoform 1.18.1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Ncam1_1.18.1 (Ncam1 Mouse)
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLC-CUL4A WT-P2A-Puro

    Plasmid

    #124304

    Purpose

    Lentiviral vector for expression of wild-type CUL4A-P2A-Puro casette from a CMV promoter

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

    Insert

    CUL4A (CUL4A Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. EF1a_MESP1_P2A_Hygro_Barcode

    Plasmid

    #120459

    Purpose

    Barcoded lentiviral vector to express MESP1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    MESP1 (MESP1 Human)
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. JE301: pMVP (L5-L4) Tir1-P2A-AID

    Plasmid

    #121722

    Purpose

    pMVP L5-L4 entry plasmid, contains Tir1-P2A-AID for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term Tir1 linked by P2A to AID domain fused to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    osTir1-P2A-AID (open)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    9x myc (C-term on osTIR1)

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. FPR3-DuET

    Plasmid

    #213241

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    FPR3 (FPR3 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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