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pcDNA3.1(+)/GGG-nLuc-3XFLAG

Plasmid

#127307

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from GGG start codon (Negative control plasmid)

Depositor

Jeremy Wilusz

Publication

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pGL4.13/AUG(59)-FFLuc-3XFLAG

Plasmid

#127337

Purpose

Express truncated 3xFLAG tagged firefly luciferase (FFLuc) from AUG at codon 59

Depositor

Jeremy Wilusz

Publication

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

Firefly luciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

SV40

Available since

June 21, 2019

Availability

Academic Institutions and Nonprofits only

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pTBL322 GC

Plasmid

#126950

Purpose

To target a protospacer with GC at the cut site.

Depositor

Chang Liu

Publication

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human genome with GC at cut site

Expression

Mammalian

Promoter

human U6

Available since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

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pTBL581 GA

Plasmid

#126951

Purpose

To target a protospacer with GA at the cut site.

Depositor

Chang Liu

Publication

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human genome with GA at cut site

Expression

Mammalian

Promoter

human U6

Available since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

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pTBL601 AG

Plasmid

#126952

Purpose

To target a protospacer with AG at the cut site.

Depositor

Chang Liu

Publication

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human genome with AG at cut site

Expression

Mammalian

Promoter

human U6

Available since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

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pTBL602 CG

Plasmid

#126953

Purpose

To target a protospacer with CG at the cut site.

Depositor

Chang Liu

Publication

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human genome with CG at cut site

Expression

Mammalian

Promoter

human U6

Available since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

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pSB139 - pL0_ZCT1 (CDS1)

Plasmid

#123184

Purpose

Golden Gate (MoClo; CDS1) compatible ZCT1 gene from Catharanthus roseus; a repressor of the STR1 promoter from C. roseus

Depositor

Erin Cram, Carolyn Lee-Parsons

Publication

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

ZCT1 from Catharanthus roseus

Use

Part for plant expression

Expression

Plant

Mutation

No BpiI and BsaI sites

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pSB142 - pL0_ORCA3 (CDS1)

Plasmid

#123185

Purpose

Golden Gate (MoClo; CDS1) compatible ORCA3 gene from Catharanthus roseus; an activator of the STR1 promoter from C. roseus

Depositor

Erin Cram, Carolyn Lee-Parsons

Publication

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

ORCA3 from Catharanthus roseus

Use

Part for plant expression

Expression

Plant

Mutation

No BpiI and BsaI sites

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pJL1

Plasmid

#117429

Purpose

Expression of ARL1

Depositor

Richard Kahn

Publication

Van Valkenburgh et al J Biol Chem. 2001 Jun 22;276(25):22826-3

Insert

ARL1 (ARL1 Human)

Expression

Bacterial

Available since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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rs210-major

Plasmid

#113377

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs2104047 major allele (A)

Depositor

Ralf Jauch

Publication

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs2104047 major allele

Use

Luciferase

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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rs210-minor

Plasmid

#113378

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs2104047 minor allele (G)

Depositor

Ralf Jauch

Publication

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs2104047 minor allele

Use

Luciferase

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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rs438-major

Plasmid

#113369

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs4384382 major allele (A)

Depositor

Ralf Jauch

Publication

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs4384382 major allele

Use

Luciferase

Available since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only
rs344-minor

Plasmid

#113366

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs34466261 minor allele (A)

Depositor

Ralf Jauch

Publication

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs34466261 minor allele

Use

Luciferase

Available since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only

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rs438-minor

Plasmid

#113370

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs4384382 minor allele (G)

Depositor

Ralf Jauch

Publication

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs4384382 minor allele

Use

Luciferase

Available since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only

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rs294-minor

Plasmid

#113374

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs2941742 minor allele (G)

Depositor

Ralf Jauch

Publication

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs2941742 minor allele

Use

Luciferase

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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rs294-major

Plasmid

#113373

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs2941742 major allele (A)

Depositor

Ralf Jauch

Publication

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs2941742 major allele

Use

Luciferase

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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rs281-minor

Plasmid

#113372

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs281038 minor allele (G)

Depositor

Ralf Jauch

Publication

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs281038 minor allele

Use

Luciferase

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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rs281-major

Plasmid

#113371

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs281038 major allele (A)

Depositor

Ralf Jauch

Publication

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs281038 major allele

Use

Luciferase

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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rs117-major

Plasmid

#113367

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs11716984 major allele (A)

Depositor

Ralf Jauch

Publication

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs11716984 major allele

Use

Luciferase

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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rs117-minor

Plasmid

#113368

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs11716984 minor allele (T)

Depositor

Ralf Jauch

Publication

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs11716984 minor allele

Use

Luciferase

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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