A DDX6-CNOT1 complex and W-binding pockets in CNOT9 reveal direct links between miRNA target recognition and silencing.
Chen Y, Boland A, Kuzuoglu-Ozturk D, Bawankar P, Loh B, Chang CT, Weichenrieder O, Izaurralde E
Mol Cell. 2014 Jun 5;54(5):737-50. doi: 10.1016/j.molcel.2014.03.034. Epub 2014 Apr 24. PubMed Article
Chen Y, Boland A, Kuzuoglu-Ozturk D, Bawankar P, Loh B, Chang CT, Weichenrieder O, Izaurralde E
Mol Cell. 2014 Jun 5;54(5):737-50. doi: 10.1016/j.molcel.2014.03.034. Epub 2014 Apr 24. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 146701 | pAc5.1B-EGFP-DmNot1_1710-2505_J | Insect Expression of DmNot1_1710-2505 |
| 146702 | pAc5.1B-lambdaN-HA-DmNot1_1710-2505_J | Insect Expression of DmNot1_1710-2505 |
| 147098 | pCIneo-lambdaN-HA-HsRCK_1-295_O | Mammalian Expression of HsRck_1-295 |
| 147099 | pCIneo-lambdaN-HA-HsRCK_296-472_O | Mammalian Expression of HsRck_296-472 |
| 147480 | pLNHA-C1-HsNot9_18-285_S | Mammalian Expression of HsNot9_18-285 |
| 147481 | pnEA-NpH-HsNot9_19-285_S | Bacterial Expression of HsNot9_19-285 |
| 147482 | pT7-EGFP-C1-HsNot9_18-285_S | Mammalian Expression of HsNot9_18-285 |
| 147573 | pnEA-NpH-HsNot1iso1_1093-1317_T | Bacterial Expression of HsNot1iso1_1093-1317 |
| 147574 | pnEA-NpM-HsNot1iso1_1093-1317-K1208AH1212AK1218A_T | Bacterial Expression of HsNot1iso1_1093-1317-K1208AH1212AK1218A |
| 147773 | pnEA-NpH-HsNot9_19-285-P165AY203AR205AR244A_V | Bacterial Expression of HsNot9_19-285-P165AY203AR244A |
| 147774 | pnEA-NpH-HsNot9_19-285-R205AH208A_V | Bacterial Expression of HsNot9_19-285-R205AH208A |
| 147775 | pnEA-NpH-HsNot9_19-285-Y203AR205AH208AA248F_V | Bacterial Expression of HsNot9_19-285-Y203AR244AH208AA248F |
| 147776 | pnEA-NpH-HsNot9_19-285-Y203AR205AR244AH208A_V | Bacterial Expression of HsNot9_19-285-Y203AR244AR244AH208A |
| 147777 | pnEA-NpH-HsNot9_19-285-Y203AR244A_V | Bacterial Expression of HsNot9_19-285-Y203AR244A |
| 147778 | pnYC-NpM-HsNot1iso1_1356-1561_V | Bacterial Expression of HsNot1iso1_1356-1561 |
| 147779 | pnYC-NpM-HsNot1iso1_1356-1581_V | Bacterial Expression of HsNot1iso1_1356-1581 |
| 147780 | pnYC-NpM-HsNot1iso1_1356-1607_V | Bacterial Expression of HsNot1iso1_1356-1607 |
| 147781 | pnYC-NpM-HsNot1iso1_1356-1628_V | Bacterial Expression of HsNot1iso1_1356-1628 |
| 147829 | pAc5.1B-lambdaN-HA-DmCaf40-NFCVYAtoAAYYAF_W | Insect Expression of DmCaf40-NFCVYAtoAAYYAF |
| 147830 | pAc5.1B-lambdaN-HA-DmGW182_1-634_W | Insect Expression of DmGW182_1-634 |
| 147831 | pAc5.1B-lambdaN-HA-DmGW182_635-1384_W | Insect Expression of DmGW182_635-1384 |
| 147832 | pMT-CTAP-PrP-SBP-DmCaf40_W | Insect Expression of DmCaf40 |
| 147833 | pMT-CTAP-PrP-SBP-DmCaf40-N63AF65AC69YV76Y_W | Insect Expression of DmCaf40-N63AF65AC69YV76Y |
| 147834 | pMT-CTAP-PrP-SBP-DmCaf40-NFCVYAtoAAYYAF_W | Insect Expression of DmCaf40-NFCVYAtoAAYYAF |
| 147835 | pMT-EGFP-CTAP-PrP-SBP_W | Insect Expression of EGFP |
| 147836 | pT7-EGFP-C1-HsNot1iso1-del1097-1110_W | Mammalian Expression of HsNot1iso1-del1097-1110 |
| 147837 | pT7-EGFP-C1-HsNot1iso1-IKGIRQtoDSYDAA-shRNAres_W | Mammalian Expression of HsNot1iso1-IKGIRQtoDSYDAA-shRNAres |
| 147838 | pAc5.1B-EGFP-DmCaf40-NFCVYAtoAAYYAF_W | Insect Expression of DmCaf40-NFCVYAtoAAYYAF |
| 147839 | pAc5.1B-EGFP-DmCaf40-R210AH213A_W | Insect Expression of DmCaf40-R210AH213A |
| 147840 | pAc5.1B-EGFP-DmMe31B-Q294AN296F-dsRNAres_W | Insect Expression of DmMe31B-Q294AAN296F-dsRNAres |
| 147841 | pAc5.1B-EGFP-DmMe31B-Q294AN296FR347A-dsRNAres_W | Insect Expression of DmMe31B-Q294AAN296FR347A-dsRNAres |
| 147842 | pAc5.1B-EGFP-DmMe31B-R347A-dsRNAres_W | Insect Expression of DmMe31B-R347A-dsRNAres |
| 147843 | pAc5.1B-EGFP-DmMe31B-S291A-dsRNAres_W | Insect Expression of DmMe31B-S291A-dsRNAres |
| 147844 | pAc5.1B-EGFP-DmNot1_1-812_W | Insect Expression of DmNot1_1-812 |
| 147845 | pAc5.1B-EGFP-DmNot1_812-1945-dsRNAres_W | Insect Expression of DmNot1_812-1945-dsRNAres |
| 147846 | pAc5.1B-EGFP-DmNot1_812-1945-K1537SG1562EQ1658A-dsRNAres_W | Insect Expression of DmNot1_812-1945-K1537SG1562EQ1658A-dsRNAres |
| 147847 | pAc5.1B-EGFP-DmNot1_1387-1699_W | Insect Expression of DmNot1_1387-1699 |
| 147848 | pAc5.1B-EGFP-DmNot1_1387-1717_W | Insect Expression of DmNot1_1387-1717 |
| 147849 | pAc5.1B-EGFP-DmNot1_1467-1699_W | Insect Expression of DmNot1_1467-1699 |
| 147850 | pAc5.1B-EGFP-DmNot1_1467-1717_W | Insect Expression of DmNot1_1467-1717 |
| 147851 | pAc5.1B-EGFP-DmNot1_1467-1717-G1562E_W | Insect Expression of DmNot1_1467-1717-G1562E |
| 147852 | pAc5.1B-EGFP-DmNot1_1467-1717-G1562Y_W | Insect Expression of DmNot1_1467-1717-G1562Y |
| 147853 | pAc5.1B-EGFP-DmNot1_1467-1717-K1537S_W | Insect Expression of DmNot1_1467-1717-K1537S |
| 147854 | pAc5.1B-EGFP-DmNot1_1467-1717-K1537SG1562EQ1658A_W | Insect Expression of DmNot1_1467-1717-K1537SG1562EQ1658A |
| 147855 | pAc5.1B-EGFP-DmNot1_1467-1717-K1569A_W | Insect Expression of DmNot1_1467-1717-K1569A |
| 147856 | pAc5.1B-EGFP-DmNot1_1467-1717-Q1658A_W | Insect Expression of DmNot1_1467-1717-Q1658A |
| 147857 | pAc5.1B-EGFP-DmNot1_1497-1644_W | Insect Expression of DmNot1_1497-1644 |
| 147858 | pAc5.1B-EGFP-DmNot1_1941-2505-dsRNAres_W | Insect Expression of DmNot1_1941-2505-dsRNAres |
| 147859 | pAc5.1B-EGFP-DmNot1-del1156-1169-RNFtoAAA-dsRNAres_W | Insect Expression of DmNot1-del1156-1169-RNFtoAAA-dsRNAres |
| 147860 | pAc5.1B-EGFP-DmNot1-K1537SG1562EQ1658A-dsRNAres_W | Insect Expression of DmNot1-K1537SG1562EQ1658A-dsRNAres |
| 147861 | pAc5.1B-EGFP-DmNot1-RNFtoAAA-dsRNAres_W | Insect Expression of DmNot1-RNFtoAAA-dsRNAres |
| 147862 | pAc5.1B-HA-DmMe31B-Q294AN296F-dsRNAres_W | Insect Expression of DmMe31B-Q294AAN296F-dsRNAres |
| 147863 | pAc5.1B-HA-DmMe31B-Q294AN296FR347A-dsRNAres_W | Insect Expression of DmMe31B-Q294AAN296FR347A-dsRNAres |
| 147864 | pAc5.1B-HA-DmMe31B-R347A-dsRNAres_W | Insect Expression of DmMe31B-R347A-dsRNAres |
| 147865 | pAc5.1B-HA-DmMe31B-S291A-dsRNAres_W | Insect Expression of DmMe31B-S291A-dsRNAres |
| 147866 | pAc5.1B-lambdaN-HA-DmCaf40_25-291_W | Insect Expression of DmCaf40_25-291 |
| 147867 | pAc5.1B-lambdaN-HA-DmNot1_1467-1717-K1537S_W | Insect Expression of DmNot1_1467-1717-K1537S |
| 147868 | pAc5.1B-lambdaN-HA-DmNot1_1467-1717-K1537SG1562EQ1658A_W | Insect Expression of DmNot1_1467-1717-K1537SG1562EQ1658A |
| 147869 | pAc5.1B-lambdaN-HA-DmNot1_1467-1717-K1537SQ1658A_W | Insect Expression of DmNot1_1467-1717-K1537SQ1658A |
| 147870 | pAc5.1B-lambdaN-HA-DmNot1_1467-1717-K1569A_W | Insect Expression of DmNot1_1467-1717-K1569A |
| 147871 | pAc5.1B-lambdaN-HA-DmNot1_1467-1717-Q1658A_W | Insect Expression of DmNot1_1467-1717-Q1658A |
| 147872 | pAc5.1B-lambdaN-HA-DmNot1_1497-1644_W | Insect Expression of DmNot1_1497-1644 |
| 147873 | pAc5.1B-lambdaN-HA-DmNot1_1497-1717-G1562E_W | Insect Expression of DmNot1_1497-1717-G1562E |
| 147874 | pAc5.1B-lambdaN-HA-DmNot1_1497-1717-G1562Y_W | Insect Expression of DmNot1_1497-1717-G1562Y |
| 147875 | pAc5.1B-lambdaN-HA-DmNot1-del1156-1169-RNFtoAAA-dsRNAres_W | Insect Expression of DmNot1-del1156-1169-RNFtoAAA-dsRNAres |
| 147876 | pAc5.1B-lambdaN-HA-DmNot1-K1537SG1562EQ1658A-dsRNAres_W | Insect Expression of DmNot1-K1537SG1562EQ1658A-dsRNAres |
| 147877 | pAc5.1B-lambdaN-HA-DmNot1-K1537SQ1658A-dsRNAres_W | Insect Expression of DmNot1-K1537SQ1658A-dsRNAres |
| 147878 | pAc5.1B-lambdaN-HA-DmNot1-RNFtoAAA-dsRNAres_W | Insect Expression of DmNot1-RNFtoAAA-dsRNAres |
| 147879 | pCIneo-lambdaN-HsNot1_1318-1605-HA-MBP_W | Mammalian Expression of HsNot1iso1_1318-1605 |
| 147881 | pLNHA-C1-HsNot1iso1-del1097-1110-R1138A-shRNAres_W | Mammalian Expression of HsNot1iso1-del1097-1110-R1138A-shRNAres |
| 147882 | pLNHA-C1-HsNot1iso1-del1097-1110-shRNAres_W | Mammalian Expression of HsNot1iso1-del1097-1110-shRNAres |
| 147883 | pnYC-NpM-HsNot1iso1_1833-2361-F2353D_W | Bacterial Expression of HsNot1iso1_1833-2361-F2353D |
| 147884 | pnYC-NpM-HsNot1iso1_1833-2361-I2330E_W | Bacterial Expression of HsNot1iso1_1833-2361-I2330E |
| 147885 | pSUPERpuro-BglII-HsNot9shRNA2_W | Mammalian Expression of shRNA2-HsNot9 |
| 147886 | pT7-EGFP-C1-HsNot1iso1_1-1605-del1079-1110-RNFtoAAA-shRNAres_W | Mammalian Expression of HsNot1iso1_1-1605-del1097-1110-RNFtoAAA-shRNAres |
| 147887 | pT7-EGFP-C1-HsNot1iso1_1-1605-del1097-1110-RNFIKGRQtoAAADSYAA-shRNAres_W | Mammalian Expression of HsNot1iso1_1-1605-del1097-1110-RNFIKGRQtoAAADSYAA-shRNAres |
| 147888 | pT7-EGFP-C1-HsNot1iso1_1-1605-IKGRQtoDSYAA-shRNAres_W | Mammalian Expression of HsNot1iso1_1-1605-IKGRQtoDSYAA-shRNAres |
| 147889 | pT7-EGFP-C1-HsNot1iso1_1-1605-shRNAres_W | Mammalian Expression of HsNot1iso1_1-1605-shRNAres |
| 147890 | pT7-EGFP-C1-HsNot1iso1_1-1840-IKGRQtoDSYAA-shRNAres_W | Mammalian Expression of HsNot1iso1_1-1840-IKGRQtoDSYAA-shRNAres |
| 147891 | pT7-EGFP-C1-HsNot1iso1_1-1840-shRNAres_W | Mammalian Expression of HsNot1iso1_1-1840-shRNAres |
| 147892 | pT7-EGFP-C1-HsNot1iso1_1093-1317_W | Mammalian Expression of HsNot1iso1_1093-1317 *Please note that this plasmid encodes amino acids 1093-1322 (not 1093-1317 as the name suggests). |
| 147893 | pT7-EGFP-C1-HsNot1iso1_1318-1605_W | Mammalian Expression of HsNot1iso1_1318-1605 |
| 147894 | pT7-EGFP-C1-HsNot1iso1_1318-1605-IKGIRQtoDSYDAA_W | Mammalian Expression of HsNot1iso1_1318-1605-IKGIRQtoDSYDAA |
| 147895 | pT7-EGFP-C1-HsNot1iso1_1318-1605-IKGRQtoDSYAA_W | Mammalian Expression of HsNot1iso1_1318-1605-IKGRQtoDSYAA |
| 147896 | pT7-EGFP-C1-HsNot1iso1_1318-1605-KGRQtoSYAA_W | Mammalian Expression of HsNot1iso1_1318-1605-KGRQ-SYAA |
| 147897 | pT7-EGFP-C1-HsNot1iso1-del1097-1110-IKGRQtoDSYAA-shRNAres_W | Mammalian Expression of HsNot1iso1-del1097-1110-IKGRQtoDSYAA-shRNAres |
| 147898 | pT7-EGFP-C1-HsNot1iso1-del1097-1110-NF1144AA-shRNAres_W | Mammalian Expression of HsNot1iso1-del1097-1110-NF1144AA-shRNAres |
| 147899 | pT7-EGFP-C1-HsNot1iso1-del1097-1110-R1138A-shRNAres_W | Mammalian Expression of HsNot1iso1-del1097-1110-R1138A-shRNAres |
| 147900 | pT7-EGFP-C1-HsNot1iso1-IKGRQtoDSYAA-shRNAres_W | Mammalian Expression of HsNot1iso1-IKGRQtoDSYAA-shRNAres |
| 147901 | pT7-EGFP-C1-HsNot1iso1-KGRQtoSYAA-shRNAres_W | Mammalian Expression of HsNot1iso1-KGRQtoSYAA-shRNAres |
| 147902 | pT7-EGFP-C1-HsNot9-H58AF60AA64YV71Y-shRNA1res_W | Mammalian Expression of HsNot9-H58AF60AA64YV71Y-shRNA1res |
| 147903 | pT7-EGFP-C1-HsNot9-R205AH208A-shRNA1res_W | Mammalian Expression of HsNot9-R205AH208A-shRNA1res |
| 147904 | pT7-EGFP-C1-HsNot9-shRNAres1_W | Mammalian Expression of HsNot9-shRNAres1 |
| 147905 | pT7-EGFP-C1-HsNot9-shRNAres2_W | Mammalian Expression of HsNot9-shRNAres2 |
| 147906 | pT7-EGFP-C1-HsNot9-shRNAres2-H58AF60AA64YV71Y_W | Mammalian Expression of HsNot9-shRNAres2-H58AF60AA64YV71Y |
| 147907 | pT7-EGFP-C1-HsNot9-Y203AR205AH208AR244A-shRNA1res_W | Mammalian Expression of HsNot9-Y203AR205AH208AR244A-shRNA1res |
| 147908 | pT7-EGFP-C1-HsNot9-Y203AR244A-shRNA1res_W | Mammalian Expression of HsNot9-Y203AR244A-shRNA1res |
| 147973 | pAc5.1B-EGFP-DmCaf40-A253FY208A_X | Insect Expression of DmCaf40-A253FY208A |
| 147974 | pAc5.1B-EGFP-DmCaf40-F65AC69Y_X | Insect Expression of DmCaf40-F65AC69Y |
| 147975 | pAc5.1B-EGFP-DmCaf40-N63AF65AC69Y_X | Insect Expression of DmCaf40-N63AF65AC69Y |
| 147976 | pAc5.1B-EGFP-DmCaf40-N63AF65AC69YV76Y_X | Insect Expression of DmCaf40-N63AF65AC69YV76Y |
| 147977 | pAc5.1B-EGFP-DmCaf40-N63AF65AC69YV76YR210AH213A_X | Insect Expression of DmCaf40-N63AF65AC69YV76YR210AH213A |
| 147978 | pAc5.1B-EGFP-DmCaf40-NFCVYRHAtoAAYYAAAF_X | Insect Expression of DmCaf40-NFCVYRHAtoAAYAAAF |
| 147979 | pAc5.1B-EGFP-DmCaf40-R249H_X | Insect Expression of DmCaf40-R249H |
| 147980 | pAc5.1B-EGFP-DmCaf40-Y208AR210AH213AA253F_X | Insect Expression of DmCaf40-Y208AR210AH213AA253F |
| 147981 | pAc5.1B-EGFP-DmCaf40-Y208AR249A_X | Insect Expression of DmCaf40-Y208AR249A |
| 147982 | pAc5.1B-EGFP-DmNot1_1-1717_X | Insect Expression of DmNot1_1-1717 |
| 147983 | pAc5.1B-EGFP-DmNot1_1-1717-del1156-1169_X | Insect Expression of DmNot1_1-1717-del1156-1169 |
| 147984 | pAc5.1B-EGFP-DmNot1_1-1717-del1156-1169-R1197AN1203AF1204A_X | Insect Expression of DmNot1_1-1717-del1156-1169-R1197AN1203AF1204A |
| 147985 | pAc5.1B-EGFP-DmNot1_1-1717-del1156-1169-RNFKGQtoAAASEA_X | Insect Expression of DmNot1_1-1717-del1156-1169-RNFKGQtoAAASEA |
| 147986 | pAc5.1B-EGFP-DmNot1_1-1717-K1537SG1562EQ1658A_X | Insect Expression of DmNot1_1-1717-K1537SG1562EQ1658A |
| 147987 | pAc5.1B-EGFP-DmNot1_1-1717-R1197AN1203AF1204A_X | Insect Expression of DmNot1_1-1717-R1197AN1203AF1204A |
| 147988 | pAc5.1B-EGFP-DmNot1_1-1717-RNFKGQtoAAASEA_X | Insect Expression of DmNot1_1-1717-RNFKGQtoAAASEA |
| 147989 | pAc5.1B-EGFP-DmNot1_1-1945-dsRNAres_X | Insect Expression of DmNot1_1-1945-dsRNAres |
| 147990 | pAc5.1B-EGFP-DmNot1_1-1945-K1537SG1562EQ1658A-dsRNAres_X | Insect Expression of DmNot1_1-1945-K1537SG1562EQ1658A-dsRNAres |
| 147991 | pAc5.1B-EGFP-DmNot1-del1156-1169-dsRNAres_X | Insect Expression of DmNot1-del1156-1169-dsRNAres |
| 147992 | pAc5.1B-lambdaN-HA-DmCaf40-A253FY208A_X | Insect Expression of DmCaf40-A253FY208A |
| 147993 | pAc5.1B-lambdaN-HA-DmCaf40-F65AC69Y_X | Insect Expression of DmCaf40-F65AC69Y |
| 147994 | pAc5.1B-lambdaN-HA-DmCaf40-R249H_X | Insect Expression of DmCaf40-R249H |
| 147995 | pAc5.1B-lambdaN-HA-DmCaf40-Y208AR249A_X | Insect Expression of DmCaf40-Y208AR249A |
| 147996 | pAc5.1B-lambdaN-HA-DmNot1-G1562E-dsRNAres_X | Insect Expression of DmNot1-G1562E-dsRNAres |
| 147997 | pLNHA-C1-HsNot1iso1_840-1605_X | Mammalian Expression of HsNot1iso1_840-1605 |
| 147998 | pLNHA-C1-HsNot1iso1_840-1605-del1097-1110_X | Mammalian Expression of HsNot1iso1_840-1605-del1097-1110 |
| 147999 | pLNHA-C1-HsNot1iso1_840-1605-del1097-1110-IKGRQtoDSYAA_X | Mammalian Expression of HsNot1iso1_840-1605-del1097-1110-IKGRQtoDSYAA |
| 148000 | pLNHA-C1-HsNot1iso1_840-1605-del1097-1110-RNFtoAAA_X | Mammalian Expression of HsNot1iso1_840-1605-del1097-1110-RNFtoAAA |
| 148001 | pLNHA-C1-HsNot1iso1_840-1605-IKGRQtoDSYAA_X | Mammalian Expression of HsNot1iso1_840-1605-IKGRQtoDSYAA |
| 148475 | pAc5.1B-lambdaN-HA-DmNot1_1149-1376_AC | Insect Expression of DmNot1_1149-1376 |
| 148503 | pCIneo-lambdaN-HsNot1iso1_1318-1605-HA-MBP_AD | Mammalian Expression of HsNot1iso1_1318-1605 |