Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 2,405 results for: dcas9

Showing: 1021 - 1040 of 2405 results

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pBbdCas9S_P0826L-sgRNAflaB

Plasmid

#149648

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi flaB

Depositor

Christine Jacobs-Wagner

Publication

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAflaB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, P0826L

Available since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(-10AC2)_Psyn-sgRNArodA

Plasmid

#149660

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi rodA

Depositor

Christine Jacobs-Wagner

Publication

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNArodA

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30(-10AC2), Psyn

Available since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(-10TC)_Psyn-sgRNAmreB

Plasmid

#149595

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi mreB

Depositor

Christine Jacobs-Wagner

Publication

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAmreB

Expression

Bacterial

Promoter

PflaB, PpQE30(-10TC), Psyn

Available since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(-10TC)_Psyn-sgRNAftsI

Plasmid

#149591

Purpose

all-in-all CRISPRi vector for targeting B. burgdorferi ftsI

Depositor

Christine Jacobs-Wagner

Publication

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAftsI

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30(-10TC), Psyn

Available since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_Psyn-sgRNArodA

Plasmid

#149655

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi rodA

Depositor

Christine Jacobs-Wagner

Publication

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNArodA

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, Psyn

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(-10TC)_Psyn-sgRNArodA

Plasmid

#149657

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi rodA

Depositor

Christine Jacobs-Wagner

Publication

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNArodA

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30(-10TC), Psyn

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_PresTS-sgRNAflaB

Plasmid

#149644

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi flaB

Depositor

Christine Jacobs-Wagner

Publication

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAflaB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, PresTS

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_PresTL-sgRNAflaB

Plasmid

#149645

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi flaB

Depositor

Christine Jacobs-Wagner

Publication

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAflaB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, PresTL

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_P0026-sgRNAflaB

Plasmid

#149646

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi flaB

Depositor

Christine Jacobs-Wagner

Publication

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAflaB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, P0026

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_P0826S-sgRNAflaB

Plasmid

#149647

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi flaB

Depositor

Christine Jacobs-Wagner

Publication

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAflaB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, P0826S

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_Psyn-sgRNAftsI

Plasmid

#149649

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi ftsI

Depositor

Christine Jacobs-Wagner

Publication

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAftsI

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, Psyn

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_P0526-sgRNAftsI

Plasmid

#149650

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi ftsI

Depositor

Christine Jacobs-Wagner

Publication

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAftsI

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, P0526

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(-10TC)_Psyn-sgRNAflaB

Plasmid

#149589

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi flaB

Depositor

Christine Jacobs-Wagner

Publication

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAflaB

Expression

Bacterial

Promoter

PflaB, PpQE30(-10TC), Psyn

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pC0.018

Plasmid

#119622

Purpose

Level 0 Part. CDS

Depositor

Alistair McCormick

Publication

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

dCas9+deg-tag

Use

Synthetic Biology

Mutation

bp 331 C to A , 1237 A to C, 1284 C to T, 2121 C …

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pPD231 PPH-T2A-dCas9-NFZ-P2A-BFP

Plasmid

#249155

Purpose

Overexpression of PPH-T2A-dCas9-NFZ-P2A-BFP and blasticidin resistance gene. Contains Cp36 recombination site.

Depositor

Michael Bassik

Publication

Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi

Insert

PPH-T2A-dCas9-NFZ-P2A-mTagBFP2

Use

CRISPR; Cp36 donor dna

Expression

Mammalian

Promoter

EF1a

Available since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pXFokI-dCas9

Plasmid

#60901

Purpose

Dual Expression Vector for FokI-dCas9 and gRNA

Depositor

Bruce Conklin

Publication

Miyaoka et al Sci Rep. 2016 Mar 31;6:23549. doi: 10.10

Type

Empty backbone

Use

CRISPR

Tags

3XFLAG

Expression

Mammalian

Promoter

CBh and U6

Available since

March 31, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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iC30 PB-Zim3-dCas9-P2A-BFP_hygro

Plasmid

#236743

Purpose

Constitutive expression of Zim3-dCas9

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI)

Inserts

CAG-Zim3-NLS-dCas9-HA-P2A-2xmycNLS-tagBFP-WPRE
EF1a-hygro-WPRE

Expression

Mammalian

Promoter

CAG and EF1a

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Plas-CRISPR_reporter

Plasmid

#157658

Purpose

CRISPR reporter for mRNA quantification, integrative plasmid into NPR2 gene

Depositor

Frank Albert

Publication

Brion et al Elife. 2020 Nov 16;9. pii: 60645. doi: 1

Insert

dCAS9-VP64, mCherry, KanMX

Use

Insert storage (replicative in e. coli)

Mutation

N28D in mCherry- please see depositor comments be…

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR CRY2-TSS-guide1

Plasmid

#125428

Purpose

CRISPR-mediated repression of CRY2. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

Depositor

Jorge Ferrer

Publication

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

KRAB-dCas9-2A-BlastR

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Promoter

hPGK and U6

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR CRY2-TSS-guide3

Plasmid

#125430

Purpose

CRISPR-mediated repression of CRY2. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

Depositor

Jorge Ferrer

Publication

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

KRAB-dCas9-2A-BlastR

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Promoter

hPGK and U6

Available since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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