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A CRISPR interference platform for selective downregulation of gene expression in Borrelia burgdorferi.
Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, Jacobs-Wagner C
Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 10.1128/AEM.02519-20.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
118229pBSV2HEmpty E. coli - B. burgdorferi shuttle vector; hygromycin B resistant
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149557pKFSS1_Psyn-sgRNA500gRNA expression in B. burgdorferi, parental vector
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149558pBSV2B_Psyn-sgRNA500gRNA expression in B. burgdorferi, parental vector
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149559pBSV2H_Psyn-sgRNA500gRNA expression in B. burgdorferi, parental vector
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149560pBSV2G_Psyn-sgRNAflaBflaB gRNA expression in B. burgdorferi
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149561pBSV2H_Psyn-sgRNAflaBflaB gRNA expression in B. burgdorferi
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149562pBSV2G_Psyn-sgRNAftsIftsI gRNA expression in B. burgdorferi
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149563pBSV2H_Psyn-sgRNAftsIftsI gRNA expression in B. burgdorferi
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149566pBSV2G_Psyn-sgRNAmreBmreB gRNA expression in B. burgdorferi
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149567pBSV2H_Psyn-sgRNAmreBmreB gRNA expression in B. burgdorferi
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149568pBSV2G_Psyn-sgRNArodArodA gRNA expression in B. burgdorferi
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149569pBSV2H_Psyn-sgRNArodArodA gRNA expression in B. burgdorferi
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149570pBbdCas9K_arr2parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning
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149571pBbdCas9G_arr2parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning
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149572pBbdCas9B_arr2parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning
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149573pBbdCas9H_arr2parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning
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149574pBbdCas9S(RBSmut)parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning
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149575pBbdCas9S(RBSmut)_arr2parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning
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149576pBbdCas9S(RBSmut)_Psyn-sgRNA500parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning
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149577pBbdCas9K(RBSmut)_arr2parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning
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149578pBbdCas9G(RBSmut)_arr2parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning
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149579pBbdCas9B(RBSmut)_arr2parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning
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149580pBbdCas9H(RBSmut)_arr2parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning
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149581pBbdCas9S(-10TC)parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning
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149582pBbdCas9S(-10TC)_arr2parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning
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149583pBbdCas9S(-10TC)_Psyn-sgRNA500parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning
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149584pBbdCas9K(-10TC)_arr2parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning
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149585pBbdCas9G(-10TC)_arr2parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning
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149586pBbdCas9B(-10TC)_arr2parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning
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149587pBbdCas9H(-10TC)_arr2parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning
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149588pBbdCas9S(RBSmut)_Psyn-sgRNAflaBall-in-one CRISPRi vector for targeting B. burgdorferi flaB
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149589pBbdCas9S(-10TC)_Psyn-sgRNAflaBall-in-one CRISPRi vector for targeting B. burgdorferi flaB
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149590pBbdCas9S(RBSmut)_Psyn-sgRNAftsIall-in-one CRISPRi vector for targeting B. burgdorferi ftsI
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149591pBbdCas9S(-10TC)_Psyn-sgRNAftsIall-in-all CRISPRi vector for targeting B. burgdorferi ftsI
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149594pBbdCas9S(RBSmut)_Psyn-sgRNAmreBall-in-one CRISPRi vector for targeting B. burgdorferi mreB
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149595pBbdCas9S(-10TC)_Psyn-sgRNAmreBall-in-one CRISPRi vector for targeting B. burgdorferi mreB
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149613pBSV2_Psyn-sgRNA500gRNA expression in B. burgdorferi, parental vector
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149614pBSV2G_Psyn-sgRNA500gRNA expression in B. burgdorferi, parental vector
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149615pBSV2G_PflaBS-sgRNA500gRNA expression in B. burgdorferi, parental vector
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149616pBSV2G_PresTS-sgRNA500gRNA expression in B. burgdorferi, parental vector
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149617pBSV2G_PresTL-sgRNA500gRNA expression in B. burgdorferi, parental vector
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149618pBSV2G_P0826S-sgRNA500gRNA expression in B. burgdorferi, parental vector
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149619pBSV2G_P0826L-sgRNA500gRNA expression in B. burgdorferi, parental vector
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149620pBSV2G_P0026-sgRNA500gRNA expression in B. burgdorferi, parental vector
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149621pBSV2G_P0526-sgRNA500gRNA expression in B. burgdorferi, parental vector
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149622pBSV2G_P0031-sgRNA500gRNA expression in B. burgdorferi, parental vector
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149623pBSV2G_PflaBS-sgRNAflaBflaB gRNA expression in B. burgdorferi
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149624pBSV2G_PresTS-sgRNAflaBflaB gRNA expression in B. burgdorferi
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149625pBSV2G_PresTL-sgRNAflaBflaB gRNA expression in B. burgdorferi
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149626pBSV2G_P0826S-sgRNAflaBflaB gRNA expression in B. burgdorferi
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149627pBSV2G_P0826L-sgRNAflaBflaB gRNA expression in B. burgdorferi
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149628pBSV2G_P0026-sgRNAflaBflaB gRNA expression in B. burgdorferi
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149629pBSV2G_P0526-sgRNAflaBflaB gRNA expression in B. burgdorferi
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149630pBSV2G_P0031-sgRNAflaBflaB gRNA expression in B. burgdorferi
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149631pBSV2_Psyn-sgRNAflaBflaB gRNA expression in B. burgdorferi
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149632pBSV2_Psyn-sgRNAftsIftsI gRNA expression in B. burgdorferi
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149634pBSV2_Psyn-sgRNAmreBmreB gRNA expression in B. burgdorferi
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149635pBSV2_Psyn-sgRNArodArodA gRNA expression in B. burgdorferi
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149636pBSV2_Psyn-mCherryBbPsyn-mCherry promoter strength reporter for use in B. burgdorferi
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149637pKIKan_idCas9_Chr_centersuicide vector for knock in into the B. burgdorferi chromosome
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149638pBbdCas9Sparental, all-in-one CRISPRi vector for B. burgdorferi, empty
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149639pBbdCas9S_arr2parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning
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149640pBbdCas9S_Psyn-sgRNA500parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning
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149641pBbdCas9S_P0526-sgRNA500parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning
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149642pBbdCas9S_Psyn-sgRNAflaBall-in-one CRISPRi vector for targeting B. burgdorferi flaB
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149643pBbdCas9S_PflaBS-sgRNAflaBall-in-one CRISPRi vector for targeting B. burgdorferi flaB
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149644pBbdCas9S_PresTS-sgRNAflaBall-in-one CRISPRi vector for targeting B. burgdorferi flaB
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149645pBbdCas9S_PresTL-sgRNAflaBall-in-one CRISPRi vector for targeting B. burgdorferi flaB
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149646pBbdCas9S_P0026-sgRNAflaBall-in-one CRISPRi vector for targeting B. burgdorferi flaB
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149647pBbdCas9S_P0826S-sgRNAflaBall-in-one CRISPRi vector for targeting B. burgdorferi flaB
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149648pBbdCas9S_P0826L-sgRNAflaBall-in-one CRISPRi vector for targeting B. burgdorferi flaB
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149649pBbdCas9S_Psyn-sgRNAftsIall-in-one CRISPRi vector for targeting B. burgdorferi ftsI
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149650pBbdCas9S_P0526-sgRNAftsIall-in-one CRISPRi vector for targeting B. burgdorferi ftsI
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149653pBbdCas9S_Psyn-sgRNAmreBall-in-one CRISPRi vector for targeting B. burgdorferi mreB
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149654pBbdCas9S_P0526-sgRNAmreBall-in-one CRISPRi vector for targeting B. burgdorferi mreB
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149655pBbdCas9S_Psyn-sgRNArodAall-in-one CRISPRi vector for targeting B. burgdorferi rodA
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149656pBbdCas9S(RBSmut)_Psyn-sgRNArodAall-in-one CRISPRi vector for targeting B. burgdorferi rodA
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149657pBbdCas9S(-10TC)_Psyn-sgRNArodAall-in-one CRISPRi vector for targeting B. burgdorferi rodA
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149659pBbdCas9S(-10AC1)_Psyn-sgRNArodAall-in-one CRISPRi vector for targeting B. burgdorferi rodA
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149660pBbdCas9S(-10AC2)_Psyn-sgRNArodAall-in-one CRISPRi vector for targeting B. burgdorferi rodA
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149661pBbdCas9S(-10AC12)_Psyn-sgRNArodAall-in-one CRISPRi vector for targeting B. burgdorferi rodA
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