pBSV2H
(Plasmid
#118229)
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Purpose(Empty Backbone) Empty E. coli - B. burgdorferi shuttle vector; hygromycin B resistant
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 118229 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBSV2H
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Vector typeBacterial Expression
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Selectable markersHygromycin ; Hygro B and Rifampicin
Growth in Bacteria
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Bacterial Resistance(s)Hygromycin, 200 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/early/2018/09/21/363390 for bioRxiv preprint.
Addgene QC identified a partial duplication of the segment of the plasmid containing the HygR selectable marker. The plasmid is functional for transformation and selection in B. burgdorferi. This plasmid was functional for use as described in Takacs et al. 2018.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBSV2H was a gift from Christine Jacobs-Wagner (Addgene plasmid # 118229 ; http://n2t.net/addgene:118229 ; RRID:Addgene_118229) -
For your References section:
A CRISPR interference platform for selective downregulation of gene expression in Borrelia burgdorferi. Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, Jacobs-Wagner C. Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 10.1128/AEM.02519-20. 10.1128/AEM.02519-20 PubMed 33257311
