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pBbdCas9S_P0526-sgRNA500
(Plasmid #149641)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 149641 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBbdCas9S
  • Backbone manufacturer
    CJW lab
  • Modifications to backbone
    dCas9, lacI, empty gRNA cassette
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB 5-alpha F' Iq
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Promoter PflaB, PpQE30, P0526

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer Unknown
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBbdCas9S_P0526-sgRNA500 was a gift from Christine Jacobs-Wagner (Addgene plasmid # 149641 ; http://n2t.net/addgene:149641 ; RRID:Addgene_149641)
  • For your References section:

    A CRISPR interference platform for selective downregulation of gene expression in Borrelia burgdorferi. Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, Jacobs-Wagner C. Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 10.1128/AEM.02519-20. 10.1128/AEM.02519-20 PubMed 33257311