We narrowed to 11,625 results for: 110

1. LLP457 pGK-dCas9-Suntag-BFP

   Plasmid

   #100957

   Purpose

   BPF version of LLP339, Puro is replaced by BFP driven by EF1a, dCas9 with SungTagx10 driven by pGK, with 3xHA tag

   Depositor

   Ryan Lister

   Article

   Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

   Insert

   dCas9, SunTag array (10xGCN4)
   

   Use

   CRISPR

   Tags

   3xHA and BFP

   Expression

   Mammalian

   Mutation

   D10A, H840A for dCas9

   Available Since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. 1014 pcDNA3 T7 Akt1 K179M T308A S473A

   Plasmid

   #9031

   Depositor

   William Sellers

   Article

   Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

   Insert

   Akt1 K179M T308A S473A (AKT1 Human)
   

   Expression

   Mammalian

   Mutation

   K179M T308A S473A

   Available Since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA4-FLAG-Jhdm2a-H1122A

   Plasmid

   #38140

   Depositor

   Toru Nakano, Toshinobu Nakamura

   Article

   Nakamura et al Nature. 2012 Jun 3;486(7403):415-9. doi:

   Insert

   Jhdm2a-H1122A (Kdm3a Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   protein starts at aa115; H1122A

   Promoter

   CMV

   Available Since

   Sept. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDEST-HemmarR

   Plasmid

   #31222

   Depositor

   Yuh-Nung Jan

   Article

   Han et al Proc Natl Acad Sci U S A. 2011 Jun 7;108

   Insert

   CD4-tdTom (CD4 Fly, Human, Aequorea victoria)
   

   Tags

   CD4 and tdTomato

   Expression

   Insect

   Mutation

   Fusion of signal peptide of Drosophila Akh gene, …

   Available Since

   Oct. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV40

   Plasmid

   #101061

   Purpose

   AAV vector expressing CaMPARI2_F391W (Kd = 110nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

   Depositor

   Eric Schreiter

   Article

   Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

   Has Service

   AAV1

   Insert

   CaMPARI2-F391W
   

   Use

   AAV

   Tags

   FLAG-HA-myc and NES_his

   Promoter

   hsyn (synapsin-1)

   Available Since

   Oct. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. LentiV_Neo_POU2F3_CR

   Plasmid

   #122175

   Purpose

   Lentiviral expression plasmid of human POU2F3 cDNA (CRISPR-resistant silent mutation) with neomycin resistance gene

   Depositor

   Christopher Vakoc

   Article

   Huang et al Genes Dev. 2018 Jul 1;32(13-14):915-928.

   Insert

   POU2F3 (POU2F3 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   3 silent mutations for gRNA resistance (882g-c, 8…

   Promoter

   EFS

   Available Since

   April 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. 902 pLNCX myr HA Akt1 K179M

   Plasmid

   #9006

   Depositor

   William Sellers

   Article

   Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

   Insert

   Akt1 K179M (AKT1 Human)
   

   Use

   Retroviral

   Tags

   HA and myr

   Expression

   Mammalian

   Mutation

   K179M. kinase dead.

   Available Since

   Feb. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMJA289= pSin-EF2-TRAV10-P2A-TRBV25-Puro

   Plasmid

   #116876

   Purpose

   stable TCR expression in human T cells

   Depositor

   Marcos Alcocer

   Article

   Wang et al Biotechnol Lett. 2019 Oct;41(10):1095-11

   Insert

   TRAV10 - P2A - TRBV25 (TRAV10, TRBV25-1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1-alpha

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1(+)/GUG-DAP5-3XFLAG

   Plasmid

   #127330

   Purpose

   Express 3xFLAG tagged DAP5 protein from GUG start codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   DAP5 (EIF4G2 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pME-Sun1-2xsfGFP-6xMYC-pA (JDW 681)

    Plasmid

    #224489

    Purpose

    Gateway compatible middle entry clone containing Sun1 reporter fusion to 2 copies of sfGFP and 6 MYC tags (Nuclear envelope reporter and affinity tag)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Sun1-2xsfGFP-6xMYC-pA
    

    Use

    Gateway cloning

    Available Since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. Nxph4-2A-CreERT2 Targeting Vector

    Plasmid

    #62219

    Purpose

    Targets CreERT2 to the mouse Nxph4 stop codon

    Depositor

    Hongkui Zeng

    Article

    Harris et al Front Neural Circuits. 2014 Jul 10;8:76.

    Insert

    Nxph4-2A-CreERT2 (Nxph4 Bacteriophage, Mouse)
    

    Use

    Cre/Lox and Mouse Targeting

    Expression

    Mammalian

    Promoter

    Mouse Nxph4

    Available Since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFB-EPCP5

    Plasmid

    #228879

    Purpose

    Expression of Z-tagged LRRK2 KW using Baculoviruses

    Depositor

    Sebastian Mathea

    Article

    Raig et al bioRxiv [Preprint]. 2025 Feb 13:2024.09.

    Insert

    Leucine-rich repeat kinase 2 (LRRK2 Human)
    

    Tags

    His-Z-TEV

    Expression

    Insect

    Mutation

    Amino acids deleted 1-1846

    Available Since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. FRB-GFP-Gab1(Y628F/Y660F)

    Plasmid

    #188658

    Purpose

    FRB coupled to GFP tagged Gab1 with Shp2 binding sites mutated to Phe

    Depositor

    Andrei Karginov

    Article

    Fauser et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

    Insert

    GRB associated binding protein 1 (Gab1 Mouse)
    

    Tags

    FRB and GFP

    Expression

    Mammalian

    Mutation

    Y628F and Y660F

    Promoter

    CMV

    Available Since

    Aug. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pME Actin Vhh mNeonGreen-HA-pA (JDW 1248)

    Plasmid

    #224500

    Purpose

    Gateway compatible middle entry clone containing Actin nanobody fused to mNeonGreen with an HA tag (Visualizing actin, actin chromobody)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Actin-Vhh-mNeonGreen-HA-pA
    

    Use

    Gateway cloning

    Available Since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5/FRT/TO-DDX54-FLAG/HA

    Plasmid

    #97062

    Purpose

    Expresses DDX54-FLAG/HA (C-terminal tagged) in mammalian cells

    Depositor

    Markus Landthaler

    Article

    Milek et al Genome Res. 2017 Aug;27(8):1344-1359. do

    Insert

    DDX54 (DDX54 Human)
    

    Tags

    FLAG/HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pT2K-LRL-IRES-Luc2

    Plasmid

    #109230

    Purpose

    For Tol2 transposon-mediated stable expression of LRL and IRES-luciferase

    Depositor

    Martine Roussel

    Article

    Kawauchi et al Oncogene. 2017 Sep 14;36(37):5231-5242.

    Insert

    LRL and Luc2
    

    Expression

    Mammalian

    Promoter

    CAGGS

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLex 983-Micu2

    Plasmid

    #50372

    Purpose

    Expression in mammalian cells

    Depositor

    Vamsi Mootha

    Article

    Plovanich et al PLoS One. 2013;8(2):e55785. doi: 10.1371

    Insert

    MICU2 (MICU2 Human)
    

    Use

    Lentivirus

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

18. Lenti-sgNeo/Cre

    Plasmid

    #67594

    Purpose

    Expresses an Neomycin-targeting gRNA and Cre-recombinase

    Depositor

    Monte Winslow

    Article

    Chiou et al Genes Dev. 2015 Jul 15;29(14):1576-85. d

    Insert

    sgNeo
    

    Use

    CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. PB-Neo-TetO-Cdk12_Untagged

    Plasmid

    #127178

    Purpose

    Untagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into a dox-inducible piggybac destination vector

    Depositor

    Phil Sharp

    Article

    Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

    Insert

    Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
    

    Use

    Piggybac transposase vector

    Tags

    None

    Expression

    Mammalian

    Promoter

    TetO Promoter

    Available Since

    July 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. SYKA-c020

    Plasmid

    #175489

    Purpose

    Protein expression in bacterial cells. Tandem SH2 domains, M6-N269. Can be used for crystallography.

    Depositor

    Opher Gileadi

    Article

    Bashore et al bioRxiv. 2023 Jul 29:2023.07.28.551026.

    Insert

    SYK (SYK Human)
    

    Tags

    His6-TEV

    Expression

    Bacterial

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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