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pDS_P(aphII)-dsCsgA-Mfp3-A

Plasmid

#241781

Purpose

pBBR1MCS replicative vector for increasing biofilm attachment

Depositor

Marc Güell

Publication

Nevot et al ACS Synth Biol. 2025 Jun 22. doi: 10.102

Insert

dsCsgA-Mfp3-A

Use

Synthetic Biology

Expression

Bacterial

Mutation

WT

Available since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pDS_P(aphII)-dsCsgA-Mfp3-SP

Plasmid

#241783

Purpose

pBBR1MCS replicative vector for increasing biofilm attachment

Depositor

Marc Güell

Publication

Nevot et al ACS Synth Biol. 2025 Jun 22. doi: 10.102

Insert

dsCsgA-Mfp3-SP

Use

Synthetic Biology

Expression

Bacterial

Mutation

WT

Available since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDS_P(dsCsgA)-dsCsgA-Mfp3-SP

Plasmid

#241786

Purpose

pBBR1MCS replicative vector for increasing biofilm attachment

Depositor

Marc Güell

Publication

Nevot et al ACS Synth Biol. 2025 Jun 22. doi: 10.102

Insert

dsCsgA-Mfp3-SP

Use

Synthetic Biology

Expression

Bacterial

Mutation

WT

Available since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pDS_P(dsCsgA)-dsCsgA-Mfp3-S

Plasmid

#241785

Purpose

pBBR1MCS replicative vector for increasing biofilm attachment

Depositor

Marc Güell

Publication

Nevot et al ACS Synth Biol. 2025 Jun 22. doi: 10.102

Insert

dsCsgA-Mfp3-S

Use

Synthetic Biology

Expression

Bacterial

Mutation

WT

Available since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pDS_P(aphII)-dsCsgA-Mfp3-S

Plasmid

#241782

Purpose

pBBR1MCS replicative vector for increasing biofilm attachment

Depositor

Marc Güell

Publication

Nevot et al ACS Synth Biol. 2025 Jun 22. doi: 10.102

Insert

dsCsgA-Mfp3-S

Use

Synthetic Biology

Expression

Bacterial

Mutation

WT

Available since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-Truncated_pvc10-Cas9

Plasmid

#243734

Purpose

PVC structural operon - untargeted (truncated) tail fiber (Pvc13) - spike tip (Pvc10) loaded with Cas9 on CTD

Depositor

Feng Zhang

Publication

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

pvc13-Truncated_pvc10-Cas9

Expression

Bacterial

Available since

Sept. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-Ad5_Cas9-Pvc10

Plasmid

#243735

Purpose

PVC structural operon - tail fiber (Pvc13) retargeted with Ad5 knob - spike tip (Pvc10) loaded with Cas9 on NTD

Depositor

Feng Zhang

Publication

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

pvc13-Ad5_Cas9-Pvc10

Expression

Bacterial

Available since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-Ad5_pvc8-Cas9

Plasmid

#243737

Purpose

PVC structural operon - tail fiber (Pvc13) retargeted with Ad5 knob - spike base (Pvc8) loaded with Cas9 on CTD

Depositor

Feng Zhang

Publication

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

pvc13-Ad5_pvc8-Cas9

Expression

Bacterial

Available since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pGS-BacA-21122

Plasmid

#109174

Purpose

Plasmid for expression of N-terminally 3xFLAG-tagged proteins under the control of a polh promoter in baculovirus/insect cell systems

Depositor

Monika Golas

Publication

Golas et al Mol Biotechnol. 2018 Nov;60(11):820-832.

Type

Empty backbone

Tags

3xFLAG

Expression

Insect

Promoter

polyhedrin

Available since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pGS-BacA-21222

Plasmid

#109175

Purpose

Plasmid for expression of C-terminally 3xFLAG-tagged proteins under the control of a polh promoter in baculovirus/insect cell systems

Depositor

Monika Golas

Publication

Golas et al Mol Biotechnol. 2018 Nov;60(11):820-832.

Type

Empty backbone

Tags

3xFLAG

Expression

Insect

Promoter

polyhedrin

Available since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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p366_DG

Plasmid

#234687

Purpose

Golden gate domesticated pET28b with His10-bdNEDD8 and Avi tag

Depositor

Matthew Wohlever

Publication

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Tags

Avi tag and His10-bdNEDD8

Expression

Bacterial

Mutation

No

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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p365_DG

Plasmid

#234688

Purpose

Golden gate domesticated pET28b with His10-bdNEDD8 and ALFA

Depositor

Matthew Wohlever

Publication

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Tags

ALFA and His10-bdNEDD8

Expression

Bacterial

Mutation

No

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pHK002.6

Plasmid

#235465

Purpose

Message phagemid carrying sgRNA6 (prom. J23119, backbone pBR322)

Depositor

Yolanda Schaerli

Publication

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

sgRNA6

Use

Synthetic Biology

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-BoxB-petracrRNA

Plasmid

#207625

Purpose

Expresses BoxB-petracrRNA in mammalian cells (with a human-U6 promoter)

Depositor

Jay Shendure

Publication

Choi et al Elife. 2025 Feb 12;13:RP98110. doi: 10.7

Insert

BoxB-petracrRNA

Use

Synthetic Biology

Promoter

hU6

Available since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCfb12567

Plasmid

#219927

Purpose

PrCYC with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI

Depositor

Irina Borodina

Publication

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Promoter CYC

Expression

Yeast

Available since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pCfb12566

Plasmid

#219926

Purpose

PrDGA with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI

Depositor

Irina Borodina

Publication

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Promoter DGA

Expression

Yeast

Available since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pGG-PIP65

Plasmid

#196803

Purpose

Contains ATG8A promoter

Depositor

Marc Somssich

Publication

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

AUTOPHAGY-RELATED 8A

Use

Greengate (gg) entry vector

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAM299

Plasmid

#224258

Purpose

MCMUbHis

Depositor

Andreas Martin

Publication

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

MC-Ub-3C-His

Tags

His

Expression

Bacterial

Available since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pAM302

Plasmid

#226135

Purpose

Expression of MC-Ub-UbK48R-SH3-3C-His6

Depositor

Andreas Martin

Publication

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

MC-Ub-UbK48R-SH3-3C-His6

Expression

Bacterial

Mutation

K48R

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pICOZ-P2_AG-Tn

Plasmid

#217128

Purpose

Mammalian expression vector for expression of transposase (CMV promoter)

Depositor

Haoyi Wang

Publication

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

P2_AG transposase

Expression

Mammalian

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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