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Showing: 1 - 20 of 26663 results

  1. pGoldenGreenGate-M (pGGG-M)

    Plasmid
    #165422
    Purpose
    A Golden Gate (MoClo) compatible Level 2 acceptor binary vector based on pGreenII (Hellens et al., 2000).
    Depositor
    John Innes Centre - Crop Transformation and Genome Editing Group, Wendy Harwood
    Article
    Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.
    Type
    Empty backbone
    Use
    Synthetic Biology; Level 2 golden gate (moclo) du…
    Tags
    Expression
    Mutation
    Promoter
    Contain GG cassette and MCS with LacZ for blue/wh…
    Availability
    Academic Institutions and Nonprofits only
    165422-plasmid-map-sequence-id-331272

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  2. GreenGate

    Plasmid Kit
    #1000000036
    Purpose
    Golden Gate cloning system for rapid assembly of plant transformation constructs; Uses 6 types of pre-cloned modules: promoter, N- and C-terminal tags, coding sequence, terminator, resistance cassette
    Depositor
    Jan Lohmann
    Availability
    Academic Institutions and Nonprofits only

  3. GreenGate 2.0 Toolkit

    Plasmid Kit
    #1000000241
    Purpose
    The GreenGate 2.0 (GG2.0) toolkit enables the assembly and stacking of complex transcriptional units with the GreenGate Cloning System. It is backwards compatible with most existing GreenGate modules.
    Depositor
    Alexis Maizel
    Availability
    Academic Institutions and Nonprofits only

  4. SapTrap-SEC kit for C. elegans genome engineering

    Plasmid Kit
    #1000000109
    Purpose
    Build sgRNA and homologous repair templates that incorporate self-excising drug selection cassette (SEC), using SapTrap cloning method. Addition of AAG junction.
    Depositor
    Bob Goldstein
    Availability
    Academic Institutions and Nonprofits only

  5. pVE2_19_p40S_QfDBD_VP16AD_pFullLength_pcbC_DsRed

    Plasmid
    #154241
    Purpose
    Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with full lenght promoter pcbC
    Depositor
    Arnold Driessen
    Article
    Mozsik et al Microb Cell Fact. 2019 Nov 18;18(1):203.
    Insert
    p40S:QfDBD-VP16AD-GFP-NLS
    Use
    Synthetic Biology; Fungal expression
    Tags
    GFP-NLS; DsRed-SKL
    Expression
    Bacterial
    Mutation
    Promoter
    p40S (AN0465 A. nidulans)
    Availability
    Academic Institutions and Nonprofits only
    154241-plasmid-map-sequence-id-306043

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  6. pVE2_20_p40S_QfDBD_VP16AD_pFullLength_pcbAB_DsRed

    Plasmid
    #154242
    Purpose
    Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with full lenght promoter pcbAB
    Depositor
    Arnold Driessen
    Article
    Mozsik et al Microb Cell Fact. 2019 Nov 18;18(1):203.
    Insert
    p40S:QfDBD-VP16AD-GFP-NLS
    Use
    Synthetic Biology; Fungal expression
    Tags
    GFP-NLS; DsRed-SKL
    Expression
    Bacterial
    Mutation
    Promoter
    p40S (AN0465 A. nidulans)
    Availability
    Academic Institutions and Nonprofits only
    154242-plasmid-map-sequence-id-305991

    Enlarge

  12. pcDNA3-EngHDm

    Plasmid
    #64038
    Purpose
    Mammalian expression of TTF-1 mutant homeodomain fused to the N-terminal repressor domain of the drosophila engrailed protein (Eng)
    Depositor
    Daniel Christophe
    Article
    Christophe-Hobertus et al Mol Cell Endocrinol. 2007 Jan 29;264(1-2
    Insert
    TTF-1 (TTF1 dog)
    Use
    Tags
    Eng (N-terminal repressor domain of the drosophi…
    Expression
    Mammalian
    Mutation
    homeodomain, aa 154-226, with aa 48–50 replaced b…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only

  14. pcDNA3-EngPDm

    Plasmid
    #64040
    Purpose
    Mammalian expression of mutant paired domain of Pax8 fused to the N-terminal repressor domain of the drosophila engrailed protein (Eng)
    Depositor
    Daniel Christophe
    Article
    Christophe-Hobertus et al Mol Cell Endocrinol. 2007 Jan 29;264(1-2
    Insert
    Pax8 paired domain (Pax8 Mouse)
    Use
    Tags
    Eng (N-terminal repressor domain of the drosophi…
    Expression
    Mammalian
    Mutation
    paired domain, aa 1-147, with aa 30 to 32 changed…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only

  15. ZP11 Eng2

    Plasmid
    #16930
    Depositor
    Randall Moon
    Insert
    eng2 (eng2a Zebrafish)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
Showing: 1 - 20 of 26663 results