p026 pCS2-EnR
(Plasmid #11028)

• Depositing Lab: Ramesh Shivdasani
• Publication: Shoichet et al Proc Natl Acad Sci U S A. 2000 Apr 11. 97(8):4076-81.

Backbone

• Vector backbone: pCS2
• Backbone size w/o insert (bp): 4095
• Vector type: xenopus, mammalian, avian, and zebrafish

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: engrailed
• Alt name: engrailed repressor domain
• Species: D. melanogaster (fly)
• Insert Size (bp): 882
• Mutation: Engrailed repressor domain (bases encoding amino acids 2-296 of Drosophila engrailed)
• Entrez Gene: en (a.k.a. Dmel_CG9015, 153867_at, Apa, CG9015, Dmel\CG9015, EN, En, EnR, Eng, Engr, Engrailed, Engrailed/Invected, Es, V, en1, spa2, spt)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI? (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T3 or M13 reverse

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry