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eTC GFP beta-actin full length
(Plasmid #27123)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 27123 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    beta-actin
  • Species
    H. sapiens (human)
  • Mutation
    E237A
  • Entrez Gene
    ACTB (a.k.a. BRWS1, PS1TP5BP1)
  • Tags / Fusion Proteins
    • TC tag (N terminal on insert)
    • EGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site EcoRI (destroyed during cloning)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Note: Addgene's sequencing results indicate the presence of several nucleotide inserts (starting at bp# 2459, 2501 and 2516) and several gaps (at bp# 5352-5386) when compared to the sequence provided by the depositing laboratory. The depositing scientist confirmed that our sequencing result is correct and the differences do not affect the function of the plasmid.

The plasmid map and sequence from the depositing laboratory lists EcoRI as the 3' cloning site; however, this site was destroyed during cloning. Please see the Addgene's sequencing results for cloning details.

The beta-actin insert in the plasmids contains a E237A mutation when compared to GenBank reference sequences. This mutation was unintentional, but present in the initial clone. The plasmid functions as described in the associated publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    eTC GFP beta-actin full length was a gift from Robert Singer (Addgene plasmid # 27123 ; http://n2t.net/addgene:27123 ; RRID:Addgene_27123)
  • For your References section:

    Visualization of mRNA translation in living cells. Rodriguez AJ, Shenoy SM, Singer RH, Condeelis J. J Cell Biol. 2006 Oct 9. 175(1):67-76. 10.1083/jcb.200512137 PubMed 17030983