We narrowed to 14,243 results for: cas9

Showing: 10881 - 10900 of 14243 results

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pMK278 (mClover-Hygro)

Plasmid

#72794

Purpose

C-terminal tagging with mClover using CRIPSR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mClover-Hygro

Use

CRISPR

Tags

mClover

Expression

Mammalian

Available Since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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pNSA-ARS-CRISPR-BlastR

Plasmid

#101009

Purpose

Expresses Cas9-Nlux and sgRNA and contains CEN/ARS. CCMP537 LDSP promoter driven Blasticidin resistance gene.

Depositor

Eva Farre

Article

Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

Type

Empty backbone

Use

Algae, nannochloropsis

Available Since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pMK279 (mClover-Bsr)

Plasmid

#72795

Purpose

C-terminal tagging with mClover using CRIPSR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mClover-Bsr

Use

CRISPR

Tags

mClover

Expression

Mammalian

Available Since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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pX330 EGFR-sgRNA

Plasmid

#188633

Purpose

A human codon-optimized SpCas9 and chimeric guide RNA targeting C-terminus of human EGFR

Depositor

Jared Toettcher

Article

Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

Insert

humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.139

Plasmid

#184994

Purpose

Express Cas9-P2A-Eco1RT in human cells

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

SpCas9-P2A-Eco1RT

Tags

SV40NLS

Expression

Mammalian

Mutation

Human codon optimized RT

Promoter

TetOn-3g

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pAT9748-dCBE

Plasmid

#163008

Purpose

plasmid expressing CBE with dCas9

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

dCBE

Use

CRISPR

Tags

3xFLAG and SV40 NLS

Expression

Mammalian

Promoter

EF1-a

Available Since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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doraWT_rescue_construct

Plasmid

#190609

Purpose

Puromycin-selectable expression of Dora (CG34401) in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Dorado (CG34401 Fly)

Expression

Insect

Promoter

Actin-5c

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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PX459-sgRNA048_Grb10

Plasmid

#232934

Purpose

Expression vector for a sgRNA against the mouse Grb10 locus and SpCas9 with T2A-Puromycin resistance gene.

Depositor

Danny Reinberg

Article

Lucero et al bioRxiv, March 19, 2025

Insert

Cas9-T2A-PuroR (Grb10 S. pyogenes)

Expression

Mammalian

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pMK282 (mCherry2-Bsr)

Plasmid

#72798

Purpose

C-terminal tagging with mCherry2 using CRIPSR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mCherry2-Bsr

Use

CRISPR

Tags

mCherry2

Expression

Mammalian

Available Since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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pEn-C1.1

Plasmid

#61479

Purpose

Encodes CRISPR sgRNA for Gateway or conventional cloning of 1-3 sgRNAs into pDe-CAS9 or pCAS9-TPC

Depositor

Holger Puchta

Article

Schiml et al Plant J. 2014 Oct 18. doi: 10.1111/tpj.1

Insert

AtU6-26:sgRNA

Use

CRISPR

Expression

Plant

Promoter

U6-26

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pGEL081

Plasmid

#137890

Purpose

iSTU-CRISPR/Cas9 with OsCDPK2 intron, tRNA structure backbone

Depositor

Yong Zhang

Article

Zhong et al Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pUF-AsCpf1-pre-gRNA

Plasmid

#137849

Purpose

AsCpf1 and gRNA expression plasmid for P. falciparum with yDHODH selectable marker.

Depositor

Josh Beck

Article

Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

Insert

AsCpf1 and pre-gRNA cassette

Use

CRISPR

Available Since

April 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCfB9336 (pgRNA_II-1_NatMX)

Plasmid

#161589

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site II-1

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Insert

guiding RNA

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pUF-LbCpf1-pre-gRNA

Plasmid

#137848

Purpose

LbCpf1 and gRNA expression plasmid for P. falciparum with yDHODH selectable marker.

Depositor

Josh Beck

Article

Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

Insert

LbCpf1-NLS-FLAG and gRNA cassette

Use

CRISPR

Available Since

April 7, 2020

Availability

Academic Institutions and Nonprofits only

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pJT25_GalL_BE3

Plasmid

#143736

Purpose

Expresses BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

BE3

Use

CRISPR

Expression

Yeast

Mutation

spCas9(D10A)

Promoter

GalL

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCLV3Cas-LT0

Plasmid

#210548

Purpose

Binary vector expressing the Lineage Tracing system. Cas9 expression driven by CLV3 promoter. LT0 reporter

Depositor

Ortrun Mittelsten Scheid

Article

Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

Insert

CLV3p::spCas9::PEA3At::U6p::AAVS1gRNA::pHTR5::LT0::mCherrySSM

Expression

Plant

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pgRNAtet-phaZ

Plasmid

#169874

Purpose

Single guide RNA plasmid for Cas9 targeting phaZ in P. putida

Depositor

Brian Pfleger

Article

Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

Insert

sgRNA towards phaZ in Pseudomonas putida

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

July 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMBA323

Plasmid

#214751

Purpose

To create delta(glmS,nadE) working strains and maintaining their viability

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23105-cas9 + pBAD-λ Red genes + ptet-gRNA (pBR322ori)

Use

CRISPR

Expression

Bacterial

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pgRNAkan-glpR

Plasmid

#169873

Purpose

Single guide RNA plasmid for Cas9 targeting glpR in P. putida

Depositor

Brian Pfleger

Article

Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

Insert

sgRNA towards glpR in Pseudomonas putida

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

July 9, 2021

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR - DDX3Y sgRNA 2

Plasmid

#70657

Purpose

Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a DDX3Y targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

Depositor

David Sabatini

Article

Wang et al Science. 2015 Oct 15. pii: aac7041.

Insert

sgRNA against DDX3Y

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only