We narrowed to 14,203 results for: cas9

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pDonor-PRF1-Exon3

Plasmid

#209075

Purpose

Targeting vector for the human PRF1 locus to replace exon 3 with repaired exon 3

Depositor

Klaus Rajewsky

Publication

Li et al Sci Immunol. 2024 Feb 2;9(92):eadi0042.

Insert

PRF1-Exon3 HDRT (PRF1 Human)

Use

CRISPR

Available since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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pDonor-PRF1-T2A-cDNA

Plasmid

#209076

Purpose

Targeting vector for the human PRF1 locus to replace exon 3 with repaired cDNA

Depositor

Klaus Rajewsky

Publication

Li et al Sci Immunol. 2024 Feb 2;9(92):eadi0042.

Insert

PRF1-T2A-cDNA HDRT (PRF1 Human)

Use

CRISPR

Available since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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pHDR-PMG (pXK-1650)

Plasmid

#208858

Purpose

HDR-base selectable traffic light reporter (TLR) for helping to screen HDR-editing positive cells. Key Construct: iPuroR*-CMV-PuroR.L-mCherry-PuroR.R-GFP.

Depositor

Kun Xu

Publication

Lyu et al Int J Biol Macromol. 2023 Jul 15;243:124

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pUFlip-floxed2A-KalTA4-; gcry1:BFP -2

Plasmid

#173891

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Publication

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-KalTA4; gcry1: BFP

Use

Synthetic Biology

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pDGB3 alpha2 P35S:MS2-VP64:Tnos (GB1395)

Plasmid

#160609

Purpose

TU for the constitutive expresion of the viral coat protein MS2 fused on C-terminal with the VP64

Depositor

Diego Orzaez

Publication

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

P35S:MS2-VP64:Tnos

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

P35S

Available since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pDGB3 alpha1 U6-26-1gRNA-DFR 2.1 (GB1838)

Plasmid

#160625

Purpose

GB-cassette for the expression of a guide RNA targeting the DFR promoter with 2.1 Ms2 aptamer in the 3' of the scaffold

Depositor

Diego Orzaez

Publication

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

U6-26-1gRNA-DFR 2.1

Use

CRISPR and Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

U6-26

Available since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2 sgRNA:scaffold tetraloop MS2 aptamer SAM (GB1436)

Plasmid

#160570

Purpose

A version of scaffold sgRNA with the sequence of MS2 aptamer inside the tetraloop of the scaffold

Depositor

Diego Orzaez

Publication

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

sgRNA:scaffold tetraloop MS2 aptamer SAM

Use

CRISPR

Mutation

BsaI and BsmBI sites removed

Available since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pKLV-gRNA-Bcan-Ntrk1_4

Plasmid

#136413

Purpose

Lentiviral expression of gRNAs targeting intron 13 of murine Bcan and intron 10 of murine Ntrk1. Also constitutively expresses Puromycin linked to TagBFP.

Depositor

Massimo Squatrito

Publication

Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

Insert

U6_sgRNA(Bcan)_U6_sgRNA(Ntrk1)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pKLV-gRNA-Myb-Qk_1

Plasmid

#136415

Purpose

Lentiviral expression of gRNAs targeting intron 4 of murine Qk and intron 9 of murine Myb. Also constitutively expresses Puromycin linked to TagBFP.

Depositor

Massimo Squatrito

Publication

Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

Insert

U6_sgRNA(Myb)_U6_sgRNA(Qk)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pAAVsc.U6-sgFah.Intron9.CMV/CB-EGFP

Plasmid

#121509

Purpose

Expresses sgRNA targeting mouse Fah intron 9 (sgFah.Intron9).

Depositor

Guangping Gao

Publication

Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

Insert

sgFah.intron9

Use

AAV

Expression

Mammalian

Promoter

U6

Available since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pAAVsc.U6-sgIdua.CMV/CB-EGFP

Plasmid

#121511

Purpose

Expresses sgRNA targeting mouse Idua intron 9 (sgIdua).

Depositor

Guangping Gao

Publication

Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

Insert

sgIdua

Use

AAV

Expression

Mammalian

Promoter

U6

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAAVsc.U6-sgAspa.CMV/CB-EGFP

Plasmid

#121510

Purpose

Expresses sgRNA targeting mouse Aspa intron 2 (sgAspa).

Depositor

Guangping Gao

Publication

Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

Insert

sgAspa

Use

AAV

Expression

Mammalian

Promoter

U6

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pEJS592-pCSDest2-AcrIIC4Hpa-BFPv2-IRES

Plasmid

#113438

Purpose

Expresses type II-C anti-CRISPR protein from H. parainfluenzae and a BFP from IRES in mammalian cells

Depositor

Erik Sontheimer

Publication

Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

Inserts

Type II-C anti-CRISPR
mTagBFP2

Expression

Mammalian

Promoter

CMV

Available since

Dec. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEJS593-pCSDest2-AcrIIC5Smu-BFPv2-IRES

Plasmid

#113439

Purpose

Expresses type II-C anti-CRISPR protein from S. muelleri and a BFP from IRES in mammalian cells

Depositor

Erik Sontheimer

Publication

Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

Inserts

Type II-C anti-CRISPR
mTagBFP2

Expression

Mammalian

Promoter

CMV

Available since

Dec. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pSC25

Plasmid

#104799

Purpose

CRISPR/Cas9 2xplex gRNA targeting Medtr2g101040 (Mel1). Also expresses Cas9 from Gmubi promoter from Gmubi promoter

Depositor

Nevin Young

Publication

Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

Insert

Medtr2g101040

Use

CRISPR

Expression

Plant

Available since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pSC24

Plasmid

#104798

Purpose

CRISPR/Cas9 2xplex gRNA targeting Medtr2g101120, Medtr2g101130 (Acre2). Also expresses Cas9 from rolD promoter from AtUBQ10 promoter

Depositor

Nevin Young

Publication

Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

Insert

Medtr2g101120, Medtr2g101130

Use

CRISPR

Expression

Plant

Available since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pSC22

Plasmid

#104796

Purpose

CRISPR/Cas9 2xplex gRNA targeting Medtr2g101120, Medtr2g101130 (Acre1). Also expresses Cas9 from Gmubi promoter from AtUBQ10 promoter

Depositor

Nevin Young

Publication

Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

Insert

Medtr2g101120, Medtr2g101130

Use

CRISPR

Expression

Plant

Available since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pSC19

Plasmid

#104793

Purpose

CRISPR/Cas9 2xplex gRNA targeting Medtr2g020630 (FmoI). Also expresses Cas9 from Gmubi promoter from Gmubi promoter

Depositor

Nevin Young

Publication

Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

Insert

Medtr2g020630

Use

CRISPR

Expression

Plant

Available since

Jan. 30, 2018

Availability

Academic Institutions and Nonprofits only

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AAT_g1 gRNA

Plasmid

#86005

Purpose

plasmid vector encoding for U6-driven AAT_g1 gRNA

Depositor

Tobias Cantz

Publication

Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

Insert

pU6-AAT_g1 sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

pU6

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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AAT_g2 gRNA

Plasmid

#86006

Purpose

plasmid vector encoding for U6-driven AAT_g2 gRNA (Z-allele specific)

Depositor

Tobias Cantz

Publication

Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

Insert

pU6-AAT_g2 sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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