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pDEST17-His10-Smt3-ccdB

Plasmid

#166147

Purpose

Gateway destination vector for generation of N-terminal His10-Smt3 tagged proteins in E. coli.

Depositor

Daniel Jarosz

Publication

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Type

Empty backbone

Tags

His10-Smt3

Expression

Bacterial

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST17-His10-Smt3-ccdB-SNAP

Plasmid

#166148

Purpose

Gateway destination vector for generation of N-terminal His10-Smt3 and C-terminal SNAP tagged proteins in E. coli.

Depositor

Daniel Jarosz

Publication

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Type

Empty backbone

Tags

His10-Smt3 and SNAP

Expression

Bacterial

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST17-His10-Smt3-DDX5ΔPrD(kf)-SNAP

Plasmid

#166150

Purpose

Expression of N. furzeri DDX5ΔPrD(1-535) mutant in E. coli

Depositor

Daniel Jarosz

Publication

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Insert

DDX5ΔPrD(1-535)

Tags

His10-Smt3 and SNAP

Expression

Bacterial

Mutation

ΔPrD(1-535)

Promoter

T7

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST17-His10-Smt3-DDX5ΔIDR(kf)-SNAP

Plasmid

#166151

Purpose

Expression of N. furzeri DDX5ΔIDR(1-483) mutant in E. coli

Depositor

Daniel Jarosz

Publication

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Insert

DDX5ΔIDR(1-483)

Tags

His10-Smt3 and SNAP

Expression

Bacterial

Mutation

ΔIDR(1-483)

Promoter

T7

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pMX_mePOU

Plasmid

#206396

Purpose

Expresses mouse ePOU in mammalian cells, for retrovirus generation.

Depositor

Ralf Jauch

Publication

Tan et al Mol Biol Evol. 2021 Jun 25;38(7):2854-28

Insert

ePOU

Use

Retroviral

Expression

Mammalian

Available since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pLVTHM_mePOU_3xFlag

Plasmid

#206395

Purpose

Expresses 3xflag fused mouse ePOU in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Publication

Tan et al Mol Biol Evol. 2021 Jun 25;38(7):2854-28

Insert

ePOU

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Available since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pET28 mePOU-POU

Plasmid

#206392

Purpose

Expresses the DNA binding domain of the ePOU with a 6xHis tag for protein purification

Depositor

Ralf Jauch

Publication

Tan et al Mol Biol Evol. 2021 Jun 25;38(7):2854-28

Insert

ePOU

Tags

6xHis

Expression

Bacterial

Mutation

POU DNA binding domain only

Available since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-U6-DCK-Hygro

Plasmid

#202409

Purpose

gRNA expression vector for DCK

Depositor

Morito Kurata

Publication

Sugita et al Commun Biol. 2023 Jun 1;6(1):582. doi: 1

Insert

guide RNA (DCK Human)

Use

CRISPR and Lentiviral

Tags

Dck

Promoter

U6

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pET28 mOct4-POU

Plasmid

#206393

Purpose

Expresses the DNA binding domain of the Oct4 with a 6xHis tag for protein purification

Depositor

Ralf Jauch

Publication

Tan et al Mol Biol Evol. 2021 Jun 25;38(7):2854-28

Insert

Oct4 (Pou5f1 Mouse)

Tags

6xHis

Expression

Bacterial

Mutation

POU DNA binding domain only

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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mEos3.2trLATm2allL+A

Plasmid

#203750

Purpose

Encodes the transmembrane domain from human LAT with mEos3.2 fused on the C terminus. Residues within the transmembrane domain mutated to L and A to increase the TMD interfacial surface area with the membrane. Used as a fluorescent plasma membrane probe.

Depositor

Sarah Veatch

Publication

Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

Insert

trLATm2(allL+A) (LAT Human)

Tags

mEos3.2

Expression

Mammalian

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pegRNA_HEK4_1bp-insertion

Plasmid

#201977

Purpose

Express a pegRNA used for 1bp insertion at HEK4 site

Depositor

Scot Wolfe

Publication

Liang et al Nat Methods. 2023 Jun;20(6):898-907. doi

Insert

pegRNA_HEK4_1bp-insertion

Expression

Mammalian

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3k sgRNA-2 PX459 plasmid

Plasmid

#192256

Purpose

expressing sgRNA-2 targeting eIF3k locus closed to stop coden

Depositor

Dieter Wolf

Publication

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-2 targeting eIF3k locus closed to stop coden (EIF3K Human)

Use

CRISPR

Expression

Mammalian

Available since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3k sgRNA-1 PX459 plasmid

Plasmid

#192255

Purpose

expressing sgRNA-1 targeting eIF3k locus closed to stop coden

Depositor

Dieter Wolf

Publication

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-1 targeting eIF3k locus closed to stop coden (EIF3K Human)

Use

CRISPR

Expression

Mammalian

Available since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3f sgRNA-1 PX459 plasmid

Plasmid

#192253

Purpose

expressing sgRNA-1 targeting eIF3f locus closed to stop coden

Depositor

Dieter Wolf

Publication

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-1 targeting eIF3f locus closed to stop coden (EIF3F Human)

Use

CRISPR

Expression

Mammalian

Available since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3f sgRNA-2 PX459 plasmid

Plasmid

#192254

Purpose

expressing sgRNA-2 targeting eIF3f locus closed to stop coden

Depositor

Dieter Wolf

Publication

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-2 targeting eIF3f locus closed to stop coden (EIF3F Human)

Use

CRISPR

Expression

Mammalian

Available since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3b sgRNA-2 PX459 plasmid

Plasmid

#192250

Purpose

expressing sgRNA-2 targeting eIF3b locus closed to stop coden

Depositor

Dieter Wolf

Publication

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-2 targeting eIF3b locus closed to stop coden (EIF3B Human)

Use

CRISPR

Expression

Mammalian

Available since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3b sgRNA-1 PX459 plasmid

Plasmid

#192249

Purpose

expressing sgRNA-1 targeting eIF3b locus closed to stop coden

Depositor

Dieter Wolf

Publication

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-1 targeting eIF3b locus closed to stop coden (EIF3B Human)

Use

CRISPR

Expression

Mammalian

Available since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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RPS4X sgRNA-3 lentiCRISPR v2-Blast plasmid

Plasmid

#192233

Purpose

lentiviral vector expressing sgRNA-3 targeting eIF3 binding site of RPS4X 5'UTR

Depositor

Dieter Wolf

Publication

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-3 targeting eIF3 binding site of RPS4X 5'UTR (RPS4X Human)

Use

CRISPR and Lentiviral

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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RPS15A sgRNA-5 lentiCRISPR v2-Blast plasmid

Plasmid

#192230

Purpose

lentiviral vector expressing sgRNA-5 targeting eIF3 binding site of RPS15A 5'UTR

Depositor

Dieter Wolf

Publication

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-5 targeting eIF3 binding site of RPS15A 5'UTR (RPS15A Human)

Use

CRISPR and Lentiviral

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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RPS4X sgRNA-4 lentiCRISPR v2-Blast plasmid

Plasmid

#192234

Purpose

lentiviral vector expressing sgRNA-4 targeting eIF3 binding site of RPS4X 5'UTR

Depositor

Dieter Wolf

Publication

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-4 targeting eIF3 binding site of RPS4X 5'UTR (RPS4X Human)

Use

CRISPR and Lentiviral

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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