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opt.a_pSTC0-zeo

Plasmid

#202347

Purpose

Design opt.a (greedy optimization without distance constraints) in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

Depositor

Nicholas Gauthier

Publication

Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

Insert

opt.a

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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rw-consensus_pSTC0-zeo

Plasmid

#202349

Purpose

Control rw-consensus (reweighted consensus) in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

Depositor

Nicholas Gauthier

Publication

Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

Insert

rw-consensus

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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neg.ctrl_pSTC0-zeo

Plasmid

#202350

Purpose

Control neg. ctrl (negative control S70A) in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

Depositor

Nicholas Gauthier

Publication

Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

Insert

neg. ctrl

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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98.a_pSTC0-zeo

Plasmid

#202332

Purpose

Design 98.a in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

Depositor

Nicholas Gauthier

Publication

Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

Insert

98.a

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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98.b_pSTC0-zeo

Plasmid

#202333

Purpose

Design 98.b in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

Depositor

Nicholas Gauthier

Publication

Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

Insert

98.b

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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TR2

Plasmid

#218403

Purpose

Expresses TR2 peptide in AAV9 capsid

Depositor

Casey Maguire

Publication

Hanlon et al Molecular Therapy. 2024 Jun 5. doi.org/1

Insert

TR2 7mer (RTTASLM)

Use

AAV

Available since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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NL1

Plasmid

#218404

Purpose

NL1 insert in AAV9 capsid

Depositor

Casey Maguire

Publication

Hanlon et al Molecular Therapy. 2024 Jun 5. doi.org/1

Insert

NL1 7mer (LTTEGRR)

Use

AAV

Available since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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TH1

Plasmid

#218405

Purpose

TH1 insert in AAV9 capsid

Depositor

Casey Maguire

Publication

Hanlon et al Molecular Therapy. 2024 Jun 5. doi.org/1

Insert

TH1 7mer (HPARALP)

Use

AAV

Available since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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TP1

Plasmid

#218406

Purpose

TP1 insert in AAV9 capsid

Depositor

Casey Maguire

Publication

Hanlon et al Molecular Therapy. 2024 Jun 5. doi.org/1

Insert

TP1 7mer peptide (PKYPLLG)

Use

AAV

Available since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Ypq2-2GFP-URA

Plasmid

#207010

Purpose

Integration of 2xGFP tag at YPQ2 C terminus, use auxotrophic marker URA3(Kluyveromyces lactis) for selection. Marker for yeast vacuolar membrane.

Depositor

Zhiping Xie

Publication

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

YPQ2

Tags

2xGFP

Expression

Yeast

Available since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-DDX5(PrD)

Plasmid

#166167

Purpose

Gateway entry vector for N. furzeri DDX5-PrD(535-634) mutant

Depositor

Daniel Jarosz

Publication

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Insert

DDX5-PrD(535-634)

Use

Gateway Cloning

Mutation

PrD only 1606-1902

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-DDX5ΔPrD

Plasmid

#166164

Purpose

Gateway entry vector for N. furzeri DDX5ΔPrD(1-535) mutant

Depositor

Daniel Jarosz

Publication

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Insert

DDX5ΔPrD(1-535)

Use

Gateway Cloning

Mutation

deletion of 1606-1902

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-DDX5ΔIDR

Plasmid

#166165

Purpose

Gateway entry vector for N. furzeri DDX5ΔIDR(1-483) mutant

Depositor

Daniel Jarosz

Publication

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Insert

DDX5ΔIDR(1-483)

Use

Gateway Cloning

Mutation

deletion of 1450-1902

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-DDX5(IDR)

Plasmid

#166166

Purpose

Gateway entry vector for N. furzeri DDX5-IDR(483-634) mutant

Depositor

Daniel Jarosz

Publication

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Insert

DDX5-IDR(483-634)

Use

Gateway Cloning

Mutation

IDR only 1450-1902

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST17-His10-ccdB-SNAP

Plasmid

#166146

Purpose

Gateway destination vector for generation of N-terminal His10-tagged and C-terminal SNAP-tagged proteins in E. coli.

Depositor

Daniel Jarosz

Publication

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Type

Empty backbone

Tags

His10 and SNAP

Expression

Bacterial

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST17-His10-Smt3-ccdB

Plasmid

#166147

Purpose

Gateway destination vector for generation of N-terminal His10-Smt3 tagged proteins in E. coli.

Depositor

Daniel Jarosz

Publication

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Type

Empty backbone

Tags

His10-Smt3

Expression

Bacterial

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST17-His10-Smt3-ccdB-SNAP

Plasmid

#166148

Purpose

Gateway destination vector for generation of N-terminal His10-Smt3 and C-terminal SNAP tagged proteins in E. coli.

Depositor

Daniel Jarosz

Publication

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Type

Empty backbone

Tags

His10-Smt3 and SNAP

Expression

Bacterial

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST17-His10-Smt3-DDX5ΔPrD(kf)-SNAP

Plasmid

#166150

Purpose

Expression of N. furzeri DDX5ΔPrD(1-535) mutant in E. coli

Depositor

Daniel Jarosz

Publication

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Insert

DDX5ΔPrD(1-535)

Tags

His10-Smt3 and SNAP

Expression

Bacterial

Mutation

ΔPrD(1-535)

Promoter

T7

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST17-His10-Smt3-DDX5ΔIDR(kf)-SNAP

Plasmid

#166151

Purpose

Expression of N. furzeri DDX5ΔIDR(1-483) mutant in E. coli

Depositor

Daniel Jarosz

Publication

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Insert

DDX5ΔIDR(1-483)

Tags

His10-Smt3 and SNAP

Expression

Bacterial

Mutation

ΔIDR(1-483)

Promoter

T7

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pMX_mePOU

Plasmid

#206396

Purpose

Expresses mouse ePOU in mammalian cells, for retrovirus generation.

Depositor

Ralf Jauch

Publication

Tan et al Mol Biol Evol. 2021 Jun 25;38(7):2854-28

Insert

ePOU

Use

Retroviral

Expression

Mammalian

Available since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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