Directed Evolution of an Enhanced POU Reprogramming Factor for Cell Fate Engineering.
Tan DS, Chen Y, Gao Y, Bednarz A, Wei Y, Malik V, Ho DH, Weng M, Ho SY, Srivastava Y, Velychko S, Yang X, Fan L, Kim J, Graumann J, Stormo GD, Braun T, Yan J, Scholer HR, Jauch R
Mol Biol Evol. 2021 Jun 25;38(7):2854-2868. doi: 10.1093/molbev/msab075. PubMed Article
Tan DS, Chen Y, Gao Y, Bednarz A, Wei Y, Malik V, Ho DH, Weng M, Ho SY, Srivastava Y, Velychko S, Yang X, Fan L, Kim J, Graumann J, Stormo GD, Braun T, Yan J, Scholer HR, Jauch R
Mol Biol Evol. 2021 Jun 25;38(7):2854-2868. doi: 10.1093/molbev/msab075. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 206392 | pET28 mePOU-POU | Expresses the DNA binding domain of the ePOU with a 6xHis tag for protein purification |
| 206393 | pET28 mOct4-POU | Expresses the DNA binding domain of the Oct4 with a 6xHis tag for protein purification |
| 206395 | pLVTHM_mePOU_3xFlag | Expresses 3xflag fused mouse ePOU in mammalian cells, for lentivirus generation. |
| 206396 | pMX_mePOU | Expresses mouse ePOU in mammalian cells, for retrovirus generation. |