We narrowed to 19,744 results for: Cre

1. KA701: pMVP (L3-L2) HA tag + pA; RIP::eGFP-P2A-TETa-pA

   Plasmid

   #121808

   Purpose

   pMVP L3-L2 entry plasmid, contains HA tag-polyA + RIP-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream RIP-driven GFP-P2A-TETa

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   HA epitope tag-polyA + RIP::eGFP-P2A-TETa-polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. KB701: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; CMV promoter

   Plasmid

   #121818

   Purpose

   pMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and CMV promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. EF1a_KLF8_P2A_Hygro_Barcode

   Plasmid

   #120494

   Purpose

   Barcoded lentiviral vector to express KLF8 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   KLF8 (KLF8 Human)
   

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFUSEss-CHIg-mG3_CH2charge

   Plasmid

   #105858

   Purpose

   Expression plasmid coding for mutated heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18. Introduced mutatnions modify charge of CH2 domain.

   Depositor

   Joanna Bereta

   Article

   Klaus et al Front Immunol. 2018 May 23;9:1096. doi:

   Insert

   IgG3 heavy chain (Ighg3 Mouse)
   

   Expression

   Mammalian

   Mutation

   mutated heavy chain of mouse IgG3, muatations: Hi…

   Promoter

   hEF1-HTLV prom

   Available Since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCIneoRL-Notch1 3'UTR-mir200c-mut

   Plasmid

   #84596

   Purpose

   Renilla Luciferase reporter assay for NOTCH1 3'UTR with mutations on miR-200c binding site

   Depositor

   Yanan Yang

   Article

   Zhang et al Nat Commun. 2016 Jul 26;7:12231. doi: 10

   Insert

   NOTCH1 3'UTR (NOTCH1 Human)
   

   Use

   Luciferase

   Tags

   Renilla Luciferase

   Mutation

   Mutation on miR-200c binding site on NOTCH1 3…

   Promoter

   CMV

   Available Since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pCIneoRL-LFNG 3'UTR-mir200c-mut

   Plasmid

   #84594

   Purpose

   Renilla Luciferase reporter assay for LFNG 3'UTR with mutations on miR-200c binding site

   Depositor

   Yanan Yang

   Article

   Zhang et al Nat Commun. 2016 Jul 26;7:12231. doi: 10

   Insert

   LFNG 3'UTR (LFNG Human)
   

   Use

   Luciferase

   Tags

   Renilla Luciferase

   Mutation

   Mutation on miR-200c binding site on LFNG 3'…

   Promoter

   CMV

   Available Since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

7. Lrrc4c-AP-His

   Plasmid

   #71959

   Purpose

   Expresses the extracellular region of the LRRC4C protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Lrrc4c (Lrrc4c Mouse)
   

   Tags

   AP-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LXSN-Cx43-S279A

   Plasmid

   #60705

   Purpose

   retroviral expression of rat Cx43 S279A mutant

   Depositor

   Parmender Mehta

   Article

   Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

   Insert

   Cx43 (Gja1 Rat)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   S279A

   Promoter

   LTR

   Available Since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA-Cx43-S279/282A

   Plasmid

   #60712

   Purpose

   mammalian expression of rat Cx43 S279/282A mutant

   Depositor

   Parmender Mehta

   Article

   Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

   Insert

   Cx43 (Gja1 Rat)
   

   Expression

   Mammalian

   Mutation

   S279/282A

   Promoter

   CMV

   Available Since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA-Cx43-S279/282D

    Plasmid

    #60714

    Purpose

    mammalian expression of rat Cx43 S279/282D mutant

    Depositor

    Parmender Mehta

    Article

    Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

    Insert

    Cx43 (Gja1 Rat)
    

    Expression

    Mammalian

    Mutation

    S279/282D

    Promoter

    CMV

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

11. FNE2-A72

    Plasmid

    #44608

    Depositor

    Robert Oshima

    Article

    Galang et al J Biol Chem. 1996 Apr 5;271(14):7992-8.

    Insert

    Ets2-A72 (Ets2 Mouse)
    

    Tags

    FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    THR-72 converted to Alanine

    Promoter

    CMV

    Available Since

    April 26, 2013

    Availability

    Academic Institutions and Nonprofits only

12. KHBD00295

    Plasmid

    #34421

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG12052 (lola Fly)
    

    Use

    Gateway donor vector

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

13. KHBD00321

    Plasmid

    #34446

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG8676 (Hr39 Fly)
    

    Use

    Gateway donor vector

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

14. KHBD00050

    Plasmid

    #34212

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG11502 (svp Fly)
    

    Use

    Gateway donor vector

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

15. KHBD00060

    Plasmid

    #34221

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG9415 (Xbp1 Fly)
    

    Use

    Gateway donor vector

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

16. KHBD00012

    Plasmid

    #34174

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG8328 (HLHmdelta Fly)
    

    Use

    Gateway donor vector

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

17. mCherry-Sec61b-C1

    Plasmid

    #90994

    Purpose

    Expresses mCherry-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cells

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Nixon-Abell et al Science. 2016 Oct 28;354(6311). pii: aaf

    Insert

    mCherry-Sec61b (SEC61B Synthetic, Human)
    

    Tags

    mCherry (red fluorescence)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. InsRA-eGFP

    Plasmid

    #79795

    Purpose

    Insulin receptor isoform A with a inter-domain eGFP tag at extracellular region (between furin-like domain and transmembrane domain). For imaging insulin receptor trafficing.

    Depositor

    James Johnson

    Article

    Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

    Insert

    InsRA (INSR Human)
    

    Tags

    eGFP extracellular

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TDP-REGv2(mScarlet-FLAG) matched positive control in pTwist-CMV

    Plasmid

    #216156

    Purpose

    Expresses mScarlet regardless of TDP-43 knockdown. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

    Depositor

    Pietro Fratta

    Article

    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

    Insert

    mScarlet with C-terminal FLAG tag
    

    Expression

    Mammalian

    Available Since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Lenti‐Cas9‐2A‐Blast

    Plasmid

    #73310

    Purpose

    Lentiviral vector expressing human codon-optimized S. pyogenes FLAG-tagged Cas9 and blasticidin resistance from EFS promoter

    Depositor

    Jason Moffat

    Article

    Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EFS-NS

    Available Since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge