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pBN282

Plasmid

#86727

Purpose

Tissue-specific FLP expression plasmid for C. elegans

Depositor

Peter Askjaer

Article

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

Pelt-2::FLP D5

Expression

Worm

Available Since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pBN283

Plasmid

#86728

Purpose

Tissue-specific FLP expression plasmid for C. elegans

Depositor

Peter Askjaer

Article

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

Pdat-1::FLP D5

Expression

Worm

Available Since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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HA-BAP1-A95D-GFP

Plasmid

#78914

Purpose

Mammalian expression of BAP1 with A95D and an EGFP fusion

Depositor

Michael Hendzel

Article

Ismail et al Cancer Res. 2014 Aug 15;74(16):4282-94.

Insert

BAP1 (BAP1 Human)

Tags

EGFP and HA

Expression

Mammalian

Mutation

A95D

Promoter

CMV

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
HA-BAP1-D672G-GFP

Plasmid

#78919

Purpose

Mammalian expression of BAP1 with D672G and an EGFP fusion

Depositor

Michael Hendzel

Article

Ismail et al Cancer Res. 2014 Aug 15;74(16):4282-94.

Insert

BAP1 (BAP1 Human)

Tags

EGFP and HA

Expression

Mammalian

Mutation

D672G

Promoter

CMV

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
HA-BAP1-S63C-GFP

Plasmid

#78911

Purpose

Mammalian expression of BAP1 with S63E and an EGFP fusion

Depositor

Michael Hendzel

Article

Ismail et al Cancer Res. 2014 Aug 15;74(16):4282-94.

Insert

BAP1 (BAP1 Human)

Tags

EGFP and HA

Expression

Mammalian

Mutation

S63C

Promoter

CMV

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
pU6_(GLuc)_TOP1

Plasmid

#68423

Purpose

Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. U6 promoter

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

TOP1 construct

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

human U6

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pmalc4x_E323K_TEV

Plasmid

#47699

Purpose

Expresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation E323K

Depositor

Raquel Lieberman

Article

Burns et al Biochemistry. 2011 Jul 5;50(26):5824-33.

Insert

Maltose Binding Protein and Olfactomedin-like domain with TEV cleavage site (MYOC Human)

Tags

MBP

Expression

Bacterial

Mutation

changed glutamic acid 323 to lysine

Promoter

Ptac

Available Since

Sept. 9, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pCALNL-tdTomato

Plasmid

#125573

Purpose

Cre-dependent expression of tdTomato in mammalian cells.

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

tdTomato

Use

Cre/Lox

Expression

Mammalian

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-iCre

Plasmid

#125577

Purpose

Expresses iCre under the CAG promoter in mammalian cells.

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

iCre

Expression

Mammalian

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pFastBac1-Ls-AChBP

Plasmid

#63126

Purpose

Expression of Lymnaea stagnalis AChBP

Depositor

Titia Sixma

Article

Celie et al Nat Struct Mol Biol. 2005 Jul;12(7):582-

Insert

AChBP

Expression

Insect

Promoter

Polyhedrin

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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PLEXIN A1

Plasmid

#58237

Purpose

Riboprobe for in vitro transcription of Plexin A1

Depositor

Christiana Ruhrberg

Article

Bouvree et al Circ Res. 2012 Aug 3;111(4):437-45. doi:

Insert

Plexin A1 (Plxna1 Mouse)

Expression

Bacterial

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX-TEVsite-human Glomulin

Plasmid

#52292

Purpose

bacterial expression of human Glomulin

Depositor

Brenda Schulman

Article

Duda et al Mol Cell. 2012 Aug 10;47(3):371-82. doi:

Insert

Glomulin (GLMN Human)

Tags

GST and TEV

Expression

Bacterial

Promoter

tac

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-OptoFGFR1-Y766F

Plasmid

#59777

Purpose

Expresses optoFGFR1 with mutation in PLC-gamma binding phosphotyrosine site of FGFR1

Depositor

Won Do Heo

Article

Kim et al Chem Biol. 2014 Jul 17;21(7):903-12. doi

Insert

OptoFGFR1-Y766F (FGFR1 Human, Mustard Weed)

Tags

mCitrine

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pUB6-CDC34-HA (pRKB261)

Plasmid

#99145

Purpose

Mammalian expression construct to express HA-tagged CDC34

Depositor

Robert Bradley

Article

Feng et al Mol Cell. 2017 Jul 20;67(2):239-251.e6.

Insert

CDC34 (CDC34 Human)

Tags

HA

Expression

Mammalian

Promoter

pTight

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pMyc-C1-Cep123 (CCDC123/Cep89)

Plasmid

#69836

Purpose

This plasmid encodes Cep123 (CCDC123/Cep89) isoform 1 with an N-ter Myc-tag

Depositor

Michel Bornens

Article

Sillibourne et al Biol Open. 2013 Apr 9;2(6):535-45. doi:

Insert

Cep123 (CEP89 Human)

Tags

Myc tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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Cry2 mCherry PGK-1 in pcDNA3.1

Plasmid

#64214

Purpose

mCherry fused between Cry2 and PGK-1 to study cell migration

Depositor

Narasimhan Gautam

Article

O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

Inserts

Cry2 PHR (CRY2 Mustard Weed)
PGK1 (PGK1 Human)

Tags

mcherry

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only
Cry2 mCherry GRK3 ct in pcDNA3.1

Plasmid

#64213

Purpose

mCherry fused between Cry2 and GRK3 ct to study cell migration

Depositor

Narasimhan Gautam

Article

O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

Inserts

Cry2 PHR (CRY2 Mustard Weed)
GRK3 ct (GRK3 Human)

Tags

mcherry

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only
GFP-Drosha1-850

Plasmid

#62522

Purpose

C-terminal aa851-1374 of Drosha is deleted

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with aa 851-1374 deleted (DROSHA Human)

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 851-1374

Available Since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
pAAV-TRE-DIO-hM4Di-mCherry

Plasmid

#182532

Purpose

This plasmid is for use with neuronal cell-type selective activity tagging. The hM4Di receptor expression requires both neural activity and Cre recombinase.

Depositor

William Wisden

Article

Yu et al Science. 2022 Jul;377(6601):63-72. doi:

Insert

AAV transgene - hSyn promoter-tet-flex-hM4Di-cherry

Use

AAV, Cre/Lox, and Mouse Targeting

Tags

hM4Di-mCherry

Expression

Mammalian

Promoter

tetO promoter

Available Since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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MSCV KSR2 IRES GFP

Plasmid

#25969

Depositor

Rob Lewis

Article

Costanzo-Garvey et al Cell Metab. 2009 Nov . 10(5):366-78.

Insert

Kinase suppressor of Ras 2 (Ksr2 Mouse)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Aug. 24, 2010

Availability

Academic Institutions and Nonprofits only