159,258 results

1. mNeonGreen-APEX3

   Plasmid

   #202590

   Purpose

   Gamma-retroviral vector that expresses mNeonGreen fused to APEX3.

   Depositor

   Reuben Harris

   Article

   Becker et al J Mol Biol. 2023 May 12:168145. doi: 10.

   Insert

   mNeonGreen-APEX3-V5 (APX1 )
   

   Use

   Retroviral

   Tags

   V5 epitope and mNeonGreen

   Expression

   Mammalian

   Mutation

   APEX2 L242A

   Promoter

   MESV LTR

   Available since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3_Sec61B-V5-APEX2

   Plasmid

   #83411

   Purpose

   Expressed C-term tagged APEX2 on Sec61B in mammalian cell

   Depositor

   Hyun-Woo Rhee

   Article

   Lee et al Cell Rep. 2016 May 11. pii: S2211-1247(1

   Insert

   Sec61B-V5-APEX2 (SEC61B Human, Synthetic)
   

   Use

   Tags

   APEX2 and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-F

   Available since

   March 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. PRDM1

   Plasmid

   #25249

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   PRDM1_04 (PRDM1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 13, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

4. pEGFP-N2-COL1A1

   Plasmid

   #66602

   Purpose

   Expresses procollagen 1 alpha 1 as a GFP fusion

   Depositor

   David Stephens

   Article

   Stephens et al J Cell Sci. 2002 Mar 15;115(Pt 6):1149-6

   Insert

   COL1A1 (COL1A1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLX304 YAP1

   Plasmid

   #193689

   Purpose

   Constitutive lentiviral expression of YAP1

   Depositor

   William Hahn

   Article

   Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

   Insert

   YAP1 (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-fLuc=tdT

   Plasmid

   #120870

   Purpose

   Piggybac transposon plasmid for live animal optical imaging

   Depositor

   Jordan Green

   Article

   Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

   Insert

   pcDNA3-fLuc=tdT
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTU1-A-SP44-sfGFP

   Plasmid

   #163757

   Purpose

   Expresses Streptomyces codon-optimised sfGFP

   Depositor

   Paul Freemont, Simon Moore

   Article

   Moore et al ACS Synth Biol. 2021 Feb 19;10(2):402-41

   Insert

   sfGFP
   

   Use

   Synthetic Biology

   Tags

   None

   Expression

   Bacterial

   Mutation

   Promoter

   SP44

   Available since

   Aug. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pReporter_12

   Plasmid

   #60572

   Purpose

   A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   Reporter_12
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   BBa_J23119

   Available since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. NPY-mCherry

   Plasmid

   #67156

   Purpose

   Expresses NPY-mCherry

   Depositor

   Wolfhard Almers

   Article

   Barg et al Proc Natl Acad Sci U S A. 2010 Nov 30;10

   Insert

   NPYmCherry (NPY Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   cmv

   Available since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. FUW-TetON-GFP

    Plasmid

    #115495

    Purpose

    Lentiviral plasmid for tet-inducible expression of GFP (generated from plasmid #20321)

    Depositor

    Catherine Verfaillie

    Article

    Garcia-Leon et al Stem Cell Reports. 2018 Feb 13;10(2):655

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330S-7

    Plasmid

    #58783

    Purpose

    Expresses Cas9 nuclease and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 nuclease
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. His-TEV-EGFP-PRC1

    Plasmid

    #167540

    Purpose

    For expression of His6-TEV-EGFP-PRC1 in bacteria.

    Depositor

    Tarun Kapoor

    Article

    Subramanian et al Cell. 2013 Jul 18;154(2):377-90. doi: 10

    Insert

    PRC1 (PRC1 Human)
    

    Use

    Tags

    EGFP and His6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCR4-24XPP7SL

    Plasmid

    #31864

    Depositor

    Robert Singer

    Article

    Larson et al Science. 2011 Apr 22;332(6028):475-8.

    Insert

    24xPP7 Stem Loop Cassette
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 31, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENN.AAV.hSyn.TurboRFP.WPRE.RBG

    Plasmid

    #105552

    Purpose

    AAV expression of TurboRFP from hSyn promoter

    Depositor

    James M. Wilson

    Has Service

    AAV1

    Insert

    TurboRFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. Flag-GFP11-pDEST-N

    Plasmid

    #118366

    Purpose

    Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible N-terminal vector with BiFC-tag (GFP11-Flag) to screen interaction partners.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Type

    Empty backbone

    Use

    Destination

    Tags

    GFP11-Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. p15A-OptoCre-knt-P**-R

    Plasmid

    #189851

    Purpose

    Cre-inducible activation of knt for kanamycin resistance.

    Depositor

    Mary Dunlop

    Article

    Sheets et al Nat Commun. 2023 Feb 23;14(1):1034. doi:

    Insert

    P**-loxP-TT-loxP-R-knt
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P** (modified T7 phage gene 10)

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. p15A-OptoCre-cat-P-R

    Plasmid

    #189853

    Purpose

    Cre-inducible activation of cat for chloramphenicol resistance.

    Depositor

    Mary Dunlop

    Article

    Sheets et al Nat Commun. 2023 Feb 23;14(1):1034. doi:

    Insert

    P-loxP-TT-loxP-R-cat
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P (T7 phage gene 10)

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCW57-RFP-P2A-MCS (Neo)

    Plasmid

    #89182

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo RFP using the P2A self-cleaving peptide.

    Depositor

    Adam Karpf

    Article

    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:

    Type

    Empty backbone

    Use

    Lentiviral; Doxycycline inducible; p2a self cleav…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. Human SERCA2a (pcDNA3.1+)

    Plasmid

    #75187

    Purpose

    Mammalian expression of ATP2A2

    Depositor

    Jonathan Lytton, David MacLennan

    Article

    Lytton et al J Biol Chem. 1988 Oct 15;263(29):15024-3

    Insert

    ATP2A2 (ATP2A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. CMV-B-GECO1

    Plasmid

    #32448

    Purpose

    Mammalian expression of blue fluorescent genetically encoded Ca2+ indicator for optical imaging

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    B-GECO1.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    GCaMP3 S40P/L60P/D86G/K119I/L173Q/T223S/Y224H/D30…

    Promoter

    CMV

    Available since

    Sept. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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