162,993 results

1. pCEP4-ZM197M-gp160

   Plasmid

   #123226

   Purpose

   Mammalian expression plasmid for Env from the ZM197M HIV-1 isolate

   Depositor

   Erik Procko

   Article

   Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

   Insert

   HIV-1 (ZM197M) Env
   

   Use

   Tags

   CD5 leader peptide

   Expression

   Mammalian

   Mutation

   Codon-optimized synthetic gene

   Promoter

   CMV

   Available since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. SIRT4 Flag

   Plasmid

   #13815

   Purpose

   Mammalian expression of human SIRT4 with flag tag

   Depositor

   Eric Verdin

   Article

   North et al Mol Cell. 2003 Feb . 11(2):437-44.

   Insert

   SIRT4 (SIRT4 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pB-EF1a-NLuc-IRES-Puro

   Plasmid

   #130936

   Purpose

   PiggyBac vector for constitutive NanoLuc expression in mammalian cells

   Depositor

   James Thomson

   Article

   Chu et al Cell Rep. 2019 Aug 27;28(9):2247-2255.e5

   Insert

   NanoLuc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1.Gαs-LgB113

   Plasmid

   #134362

   Purpose

   Nanoluc complementation assay. Expression of Gαs protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 113 and 114 of Gαs. Addition of the HA epitope at N terminus of Gαs.

   Depositor

   Julien Hanson

   Article

   Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

   Insert

   Gαs-LgB113 (GNAS Human)
   

   Use

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   T7

   Available since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. Red Glifon 300

   Plasmid

   #163115

   Purpose

   Red fluorescent protein-based glucose sensor for mammalian cells with higer affinity

   Depositor

   Takashi Tsuboi

   Article

   Mita et al Cell Chem Biol. 2021 Jun 29. pii: S2451-

   Insert

   Red Glifon 300
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-TRE-DIO-FLPo

   Plasmid

   #118027

   Purpose

   AAV vector for sparse and bright labeling in a cell-type specific manner

   Depositor

   Minmin Luo

   Article

   Lin et al Nat Methods. 2018 Nov 19. pii: 10.1038/s

   Insert

   TRE-DIO-FLPo
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   TRE

   Available since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMXs-ms-Klf2

   Plasmid

   #50786

   Purpose

   retroviral expression of mouse Klf2

   Depositor

   Shinya Yamanaka

   Article

   Nakagawa et al Nat Biotechnol. 2008 Jan;26(1):101-6. Ep

   Insert

   Klf2 (Klf2 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRSFDuet-1_VNp15-mNeongreen

   Plasmid

   #182389

   Purpose

   Bacterial expression of Vesicle Nucleating peptide15-mNeongreen fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp15-mNeongreen-His
   

   Use

   Tags

   Vesicle Nucleating peptide (VNp)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBEN276

   Plasmid

   #69150

   Purpose

   Integration of luciferase operon in Tn7 insertion site of Gram negative bacteria

   Depositor

   Pierre Germon

   Article

   Howe et al BMC Microbiol. 2010 Jul 23;10:197. doi:

   Insert

   luxCDABE
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   frr

   Available since

   Dec. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-SpRYc-ABE8e-EGFP

    Plasmid

    #208340

    Purpose

    Expresses SpRYc-ABE8e in mammalian cells

    Depositor

    Pranam Chatterjee

    Article

    Zhao et al Nat Commun. 2023 Oct 4;14(1):6175. doi:

    Insert

    ecTadA(8e)-nSpRYc
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Sc++ (1-1119), SpRY (1111-1368), D10A

    Promoter

    CMV

    Available since

    Aug. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP VAMP3

    Plasmid

    #42310

    Depositor

    Thierry Galli

    Article

    Galli et al Mol Biol Cell. 1998 Jun;9(6):1437-48.

    Insert

    VAMP 3 (VAMP3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. ABCA1 promoter luciferase reporter (P1 fragment)

    Plasmid

    #86443

    Purpose

    Human ABCA1 promoter luciferase reporter (P1 fragment)

    Depositor

    David Mu

    Article

    Lai et al Oncogene. 2018 Mar 19. pii: 10.1038/s413

    Insert

    Human ABCA1 (ABCA1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGWT35S-Citrine-NLS

    Plasmid

    #182583

    Purpose

    Transient expression of Citrine-NLS in plant cell (Nucleus)

    Depositor

    Yutaka Kodama

    Article

    Osaki et al PeerJ. 2017 Sep 7;5:e3779. doi: 10.7717/

    Insert

    NLS
    

    Use

    Tags

    Citrine

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S promoter

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBOB-CAG-SARS-CoV2-Spike-HA

    Plasmid

    #141347

    Purpose

    3. Generation lentiviral vector expressing the codon optimized SARS-CoV2 Spike Glycoprotein ORF with a C-terminal HA tag generated by Junko Ogawa & Gerald M Pao

    Depositor

    Gerald Pao

    Article

    Ogawa et al bioRxiv. 2020 Jul 22. doi: 10.1101/2020.

    Insert

    SARS-CoV2 Spike Glycoprotein (S SARS-CoV2 hCoV19_USA EPI_ISL_414366 (GISAID))
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    resynthesized with human codon optimization nucle…

    Promoter

    CAG

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-32a(+)-TGFB3

    Plasmid

    #120288

    Purpose

    Produce human recombinant TGFB3 in DE3 cells

    Depositor

    Paul Burridge

    Article

    Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

    Insert

    Transforming Growth Factor-β3 (TGFB3 Human, Synthetic)
    

    Use

    Tags

    6x His and S-Tag

    Expression

    Bacterial

    Mutation

    Codon optimized for E. Coli production

    Promoter

    T7 promoter

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFH2.10_Gag-MCP-P2A-eGFP

    Plasmid

    #205525

    Purpose

    Express HIV Gag-MCP-P2A-eGFP

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    Gag-MCP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pD649-HAsp-VSTM2L-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156582

    Purpose

    Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    VSTM2L (VSTM2L Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSNAP-tag (T7) Vector

    Plasmid

    #101137

    Purpose

    Expression of protein of interest with SNAP-Tag in bacterial cells

    Depositor

    New England Biolabs, Ana Egana

    Type

    Empty backbone

    Use

    Tags

    SNAP-tag (SNAP26b)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pDEST-ERBB2-v2

    Plasmid

    #154895

    Purpose

    Expresses ERBB2 (a.k.a HER2) fused to Venus fragment 2 (v2) for use in bimolecular fluorescence complementation (BiFC) assays

    Depositor

    Johanna Ivaska

    Article

    Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

    Insert

    ERBB2 (ERBB2 Human)
    

    Use

    Tags

    Venus fragment 2 (v2)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPyPGK-EGFP-TM-IRES-Pac

    Plasmid

    #183604

    Purpose

    Mammalian expression vector for membrane-tethered EGFP from the mouse Pgk1 promoter. Confers puromycin resistance.

    Depositor

    Sally Lowell

    Article

    Malaguti et al Development. 2022 May 26. pii: 275525. d

    Insert

    EGFP-TM
    

    Use

    Tags

    Human PDGFRB transmembrane domain and Mouse IgGK …

    Expression

    Mammalian

    Mutation

    Promoter

    Mouse Pgk1

    Available since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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