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pRluc-LC3wt
(Plasmid #105002)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 105002 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 105002-D50 50 μg of DNA in Tris buffer $495
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DNA 105002-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 5300
  • Modifications to backbone
    Rluc-LC3wt ORF inserted instead of the EGFP ORF.
  • Vector type
    Mammalian Expression, Luciferase
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    LC3B
  • Alt name
    MAP1LC3B
  • Species
    R. norvegicus (rat)
  • Entrez Gene
    Map1lc3b (a.k.a. LC3B, Map1lc3, Mpl3, zbs559)
  • Tag / Fusion Protein
    • Rluc with C124A substitution (N terminal on insert)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The gene was originally cloned in Tamotsu Yoshimori Laboratory, reference Kabeya et al. 2000 and addgene ID 21073.

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRluc-LC3wt was a gift from Marja Jããttelã (Addgene plasmid # 105002 ; http://n2t.net/addgene:105002 ; RRID:Addgene_105002)

  • For your References section:

    Renilla Luciferase-LC3 Based Reporter Assay for Measuring Autophagic Flux. Farkas T, Jaattela M. Methods Enzymol. 2017;588:1-13. doi: 10.1016/bs.mie.2016.09.072. Epub 2016 Nov 12. 10.1016/bs.mie.2016.09.072 PubMed 28237095
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