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pRluc-LC3G120A
(Plasmid #105003)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 105003 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 5300
  • Modifications to backbone
    Rluc-LC3G120A ORF inserted instead of the EGFP ORF.
  • Vector type
    Mammalian Expression, Luciferase
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    LC3B
  • Alt name
    MAP1LC3B
  • Species
    R. norvegicus (rat)
  • Mutation
    Glycine 120 mutated to alanine, prevents lipidation of the protein and thereby its binding to autophagosomal membranes
  • Entrez Gene
    Map1lc3b (a.k.a. LC3B, Map1lc3, Mpl3, zbs559)
  • Tag / Fusion Protein
    • Rluc with C124A substitution (N terminal on insert)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The gene was originally cloned in Tamotsu Yoshimori Laboratory, reference Kabeya et al. 2000 and addgene ID 21073.
  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRluc-LC3G120A was a gift from Marja Jããttelã (Addgene plasmid # 105003 ; http://n2t.net/addgene:105003 ; RRID:Addgene_105003)
  • For your References section:

    Renilla Luciferase-LC3 Based Reporter Assay for Measuring Autophagic Flux. Farkas T, Jaattela M. Methods Enzymol. 2017;588:1-13. doi: 10.1016/bs.mie.2016.09.072. Epub 2016 Nov 12. 10.1016/bs.mie.2016.09.072 PubMed 28237095