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Intersubunit Crosstalk in the Rag GTPase Heterodimer Enables mTORC1 to Respond Rapidly to Amino Acid Availability.
Shen K, Choe A, Sabatini DM
Mol Cell. 2017 Oct 17. pii: S1097-2765(17)30704-9. doi: 10.1016/j.molcel.2017.09.026.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
99641pETDuet RagA-RagCBacterial expression of wildtype Rag GTPase dimer
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99642pETDuet RagA(D130N)-RagCBacterial expression of RagA(D130N)-RagC
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99643pETDuet RagA-RagC(D181N)Bacterial expression of RagA-RagC(D181N)
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99644pETDuet RagA(T21N)-RagCBacterial expression of RagA(T21N)-RagC
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99645pETDuet RagA-RagC(S75N)Bacterial expression of RagA-RagC(S75N)
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99646pETDuet RagA(R37P)-RagCBacterial expression of RagA(R37P)-RagC
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99647pETDuet RagA-RagC(L91P)Bacterial expression of RagA-RagC(L91P)
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99648pETDuet RagA-RagC(K84T))Bacterial expression of RagA-RagC(K84T)
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99649pK RagA-RagCBacterial expression of wildtype Rag GTPase dimer
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99650pK RagA(T21N)-RagCBacterial expression of RagA(T21N)-RagC
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99699pK RagA-RagC(S75N)Bacterial expression of RagA-RagC(S75N)
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99700pK RagA(Q66L)-RagCBacterial expression of RagA(Q66L)-RagC
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99701pK RagA-RagC(Q120L)Bacterial expression of RagA-RagC(Q120L)
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99702pK RagA(Q66L)-RagC(Q120L)Bacterial expression of RagA(Q66L)-RagC(Q120L)
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99706pK RagA(R37P)-RagCBacterial expression of RagA(R37P)-RagC
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99707pK RagA-RagC(L91P)Bacterial expression of RagA-RagC(L91P)
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99708pK RagA-RagC(K84T)Bacterial expression of RagA-RagC(K84T)
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99709pRK5 HA-Nprl2Mammalian expression of HA-Nprl2
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99710pRK5 HA-RagAMammalian expression of HA-RagA
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99711pRK5 HA-RagA(T21N)Mammalian expression of HA-RagA(T21N)
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99712pRK5 HA-RagA(Q66L)Mammalian expression of HA-RagA(Q66L)
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99713pRK5 HA-RagA(R37P)Mammalian expression of HA-RagA(R37P)
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99714pRK5 Flag-RagAMammalian expression of Flag-RagA
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99715pRK5 Flag-RagA(T21N)Mammalian expression of Flag-RagA(T21N)
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99716pRK5 Flag-RagA(Q66L)Mammalian expression of Flag-RagA(Q66L)
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99717pRK5 Flag-RagA(R37P)Mammalian expression of Flag-RagA(R37P)
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99718pRK5 HA-RagCMammalian expression of HA-RagC
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99719pRK5 HA-RagC(S75N)Mammalian expression of HA-RagC(S75N)
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99720pRK5 HA-RagC(Q120L)Mammalian expression of HA-RagC(Q120L)
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99721pRK5 HA-RagC(L91P)Mammalian expression of HA-RagC(L91P)
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99722pRK5 HA-RagC(K84T)Mammalian expression of HA-RagC(K84T)
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99723pRK5 Flag-RagCMammalian expression of Flag-RagC
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99724pRK5 Flag-RagC(S75N)Mammalian expression of Flag-RagC(S75N)
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99725pRK5 Flag-RagC(Q120L)Mammalian expression of Flag-RagC(Q120L)
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99726pRK5 Flag-RagC(L91P)Mammalian expression of Flag-RagC(L91P)
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99727pRK5 Flag-RagC(K84T)Mammalian expression of Flag-RagC(K84T)
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