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Intersubunit Crosstalk in the Rag GTPase Heterodimer Enables mTORC1 to Respond Rapidly to Amino Acid Availability.
Shen K, Choe A, Sabatini DM
Mol Cell. 2017 Oct 17. pii: S1097-2765(17)30704-9. doi: 10.1016/j.molcel.2017.09.026.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
99641pETDuet RagA-RagCBacterial expression of wildtype Rag GTPase dimer
99642pETDuet RagA(D130N)-RagCBacterial expression of RagA(D130N)-RagC
99643pETDuet RagA-RagC(D181N)Bacterial expression of RagA-RagC(D181N)
99644pETDuet RagA(T21N)-RagCBacterial expression of RagA(T21N)-RagC
99645pETDuet RagA-RagC(S75N)Bacterial expression of RagA-RagC(S75N)
99646pETDuet RagA(R37P)-RagCBacterial expression of RagA(R37P)-RagC
99647pETDuet RagA-RagC(L91P)Bacterial expression of RagA-RagC(L91P)
99648pETDuet RagA-RagC(K84T))Bacterial expression of RagA-RagC(K84T)
99649pK RagA-RagCBacterial expression of wildtype Rag GTPase dimer
99650pK RagA(T21N)-RagCBacterial expression of RagA(T21N)-RagC
99699pK RagA-RagC(S75N)Bacterial expression of RagA-RagC(S75N)
99700pK RagA(Q66L)-RagCBacterial expression of RagA(Q66L)-RagC
99701pK RagA-RagC(Q120L)Bacterial expression of RagA-RagC(Q120L)
99702pK RagA(Q66L)-RagC(Q120L)Bacterial expression of RagA(Q66L)-RagC(Q120L)
99706pK RagA(R37P)-RagCBacterial expression of RagA(R37P)-RagC
99707pK RagA-RagC(L91P)Bacterial expression of RagA-RagC(L91P)
99708pK RagA-RagC(K84T)Bacterial expression of RagA-RagC(K84T)
99709pRK5 HA-Nprl2Mammalian expression of HA-Nprl2
99710pRK5 HA-RagAMammalian expression of HA-RagA
99711pRK5 HA-RagA(T21N)Mammalian expression of HA-RagA(T21N)
99712pRK5 HA-RagA(Q66L)Mammalian expression of HA-RagA(Q66L)
99713pRK5 HA-RagA(R37P)Mammalian expression of HA-RagA(R37P)
99714pRK5 Flag-RagAMammalian expression of Flag-RagA
99715pRK5 Flag-RagA(T21N)Mammalian expression of Flag-RagA(T21N)
99716pRK5 Flag-RagA(Q66L)Mammalian expression of Flag-RagA(Q66L)
99717pRK5 Flag-RagA(R37P)Mammalian expression of Flag-RagA(R37P)
99718pRK5 HA-RagCMammalian expression of HA-RagC
99719pRK5 HA-RagC(S75N)Mammalian expression of HA-RagC(S75N)
99720pRK5 HA-RagC(Q120L)Mammalian expression of HA-RagC(Q120L)
99721pRK5 HA-RagC(L91P)Mammalian expression of HA-RagC(L91P)
99722pRK5 HA-RagC(K84T)Mammalian expression of HA-RagC(K84T)
99723pRK5 Flag-RagCMammalian expression of Flag-RagC
99724pRK5 Flag-RagC(S75N)Mammalian expression of Flag-RagC(S75N)
99725pRK5 Flag-RagC(Q120L)Mammalian expression of Flag-RagC(Q120L)
99726pRK5 Flag-RagC(L91P)Mammalian expression of Flag-RagC(L91P)
99727pRK5 Flag-RagC(K84T)Mammalian expression of Flag-RagC(K84T)

Antibodies from Article