159,258 results

1. pJS167

   Plasmid

   #48881

   Purpose

   Positive feedback plasmid from oscillator

   Depositor

   Jeff Hasty

   Article

   Stricker et al Nature. 2008 Nov 27;456(7221):516-9. doi

   Inserts

   araC gene with ssrA tag
   monomeric yeast-enhanced green fluorescent protein
   

   Use

   Synthetic Biology

   Tags

   ssrA (TSAANDENYALAA)

   Expression

   Bacterial

   Mutation

   Promoter

   Para/lac

   Available since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pet151D-TOPO ALIX Bro1-V (1-702)

   Plasmid

   #80642

   Purpose

   Residues 1-702 of ALIX; Bacterial Expression Vector; TEV cleavable N-term His-tag; Sundquist Lab Internal ID: WISP10-648

   Depositor

   Wesley Sundquist

   Article

   McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

   Insert

   ALIX Bro1-V (PDCD6IP Human)
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   Residues 1-702

   Promoter

   Available since

   Sept. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. His-GFP-Rim2

   Plasmid

   #170869

   Purpose

   Mammalian expression of His-GFP-Rim2.

   Depositor

   Justin Taraska

   Article

   Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

   Insert

   Regulating synaptic membrane exocytosis protein 2 (Rim 2) (Rims2 Rat)
   

   Use

   Tags

   (His)6 and EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMJ117

   Plasmid

   #85997

   Purpose

   One-guide Perturb-seq vector backbone; modified human U6 promoter; constant region 3

   Depositor

   Jonathan Weissman

   Article

   Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

   Inserts

   EGFP-NT2_cr3
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   modified human U6

   Available since

   Feb. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lenti:FlipGFP-T2A-mCherry

   Plasmid

   #223198

   Purpose

   Lentiviral vector encoding FlipGFP-T2A-mCherry (w/ caspase-3 cleave site in FlipGFP for restoring GFP conformation)

   Depositor

   Yeh-Hsing Lao

   Article

   Chi et al Lab Chip. 2024 Jan 30;24(3):396-407. doi

   Insert

   FlipGFP-T2A-mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. HA-GFP1-10-pDEST-N

   Plasmid

   #118368

   Purpose

   Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible N-terminal vector with BiFC-tag (HA-GFP1-10) to screen interaction partners.

   Depositor

   Maria Vartiainen

   Article

   Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

   Type

   Empty backbone

   Use

   Destination

   Tags

   HA-GFP1-10

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-minCMV-mCherry

   Plasmid

   #27970

   Depositor

   Feng Zhang

   Article

   Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

   Insert

   mCherry
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 10, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-jAspSnFR3-mRuby3

   Plasmid

   #203498

   Purpose

   jAspSnFR3-mRuby3 in lentiviral expression vector

   Depositor

   Lucas Sullivan

   Article

   Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

   Insert

   jAspSnFR3-mRuby3
   

   Use

   Lentiviral

   Tags

   His-tag and mRuby3

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. EGFR (del3) L747-E749del, A750P

   Plasmid

   #11015

   Purpose

   Retroviral construct for expressing human EGFR with L747-E749 deletion and A750P mutation in human cells

   Depositor

   Matthew Meyerson

   Article

   Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

   Insert

   EGFR (del3) (EGFR Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   L747-E749 deleted, A750P

   Promoter

   Available since

   Dec. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

10. pRSET-mSA

    Plasmid

    #39860

    Depositor

    Sheldon Park

    Article

    Lim et al Biotechnol Bioeng. 2013 Jan;110(1):57-67

    Insert

    monomeric streptavidin
    

    Use

    Tags

    6xHis and FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. Beta-arrestin2 GFP WT

    Plasmid

    #35411

    Depositor

    Robert Lefkowitz

    Article

    Shenoy et al J Biol Chem. 2005 Apr 15;280(15):15315-2

    Insert

    βarrestin2 (Arrb2 Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28b-pchG WT

    Plasmid

    #87107

    Purpose

    expresses PchG WT, the thiazolinyl imine reductase from Pseudomonas aeruginosa

    Depositor

    Audrey Lamb

    Article

    Meneely et al Biochemistry. 2016 Sep 27;55(38):5423-33

    Insert

    pchG WT
    

    Use

    Tags

    his-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. HaloTag-ER-3

    Plasmid

    #186960

    Purpose

    ER lumen localizaing HaloTag

    Depositor

    Ke Xu

    Article

    Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

    Insert

    CALR (CALR Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Promoter

    Available since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. FASTHDR-Cterm-HaloTag-Puromycin

    Plasmid

    #167208

    Purpose

    Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with HaloTag and selection with Puromycin

    Depositor

    Oscar Perez

    Article

    Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMK286 (mAID-NeoR)

    Plasmid

    #72824

    Purpose

    C-terminal tagging with mAID using CRISPR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mAID-Neo
    

    Use

    CRISPR

    Tags

    mAID

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. eforRed chromoprotein

    Plasmid

    #117837

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses eforRed chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, eforRed
    

    Use

    Synthetic Biology; Escherichia coli

    Tags

    Expression

    Mutation

    BioBrick sites removed

    Promoter

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. p8266 LentiCRISPR v2 hygro sgNT-1

    Plasmid

    #193977

    Purpose

    Expression of spCas9 and non-targeting control sgRNA

    Depositor

    Elizabeth White

    Article

    Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

    Insert

    spCas9
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSLIK-IDH2-R172K-FLAG

    Plasmid

    #66807

    Purpose

    Tet-inducible expression of mutant IDH2 in mammalian cells

    Depositor

    Christian Metallo

    Article

    Lewis et al Mol Cell. 2014 Jul 17;55(2):253-63. doi:

    Insert

    IDH2 (IDH2 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    R172K

    Promoter

    CMV

    Available since

    Feb. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pG1C

    Plasmid

    #71740

    Purpose

    Modular shuttle vector for Geobacillus and E. coli

    Depositor

    Tom Ellis

    Article

    Reeve et al ACS Synth Biol. 2016 Jul 1.

    Type

    Empty backbone

    Use

    Synthetic Biology; Shuttle vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28b-irp3 WT

    Plasmid

    #87106

    Purpose

    expresses Irp3 WT, the thiazolinyl imine reductase from Yersinia enterocolitica

    Depositor

    Audrey Lamb

    Article

    Meneely et al Biochemistry. 2012 Nov 6;51(44):9002-13.

    Insert

    irp3 WT
    

    Use

    Tags

    his-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge