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A versatile nanobody-based toolkit to analyze retrograde transport from the cell surface
Buser DP, Schleicher KD, Prescianotto-Baschong C, Spiess M
PNAS June 18, 2018. 201801865
Article

Plasmids from Article

ID Plasmid Purpose  
109417pET24a-VHH-stdBacterial expression of a functionalized anti-GFP (VHH) nanobody. VHH-std also contains a T7, HA, BAP and His6 epitope
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109418pET24a-VHH-1xTSBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to one tyrosine sulfation (TS) motif. VHH-1xTS also contains a T7, HA, BAP and His6 epitope
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109419pET24a-VHH-2xTSBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to two tyrosine sulfation (TS) motifs. VHH-2xTS also contains a T7, HA, BAP and His6 epitope
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109420pET24a-VHH-APEX2Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to ascorbate peroxidase 2. VHH-APEX2 also contains a T7, HA, BAP and His6 epitope
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109421pET24a-VHH-mCherryBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mCherry. VHH-mCherry also contains a T7, HA, BAP and His6 epitope
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109422pET24a-VHH-mycBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to a myc epitope. VHH-myc also contains a T7, HA, BAP and His6 epitope
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109423pET24a-VHH-tev-stdBacterial expression of a functionalized anti-GFP (VHH) nanobody with a TEV site. VHH-tev-std is flanked by an N-terminal T7 epitope, and a C-terminal cassette composed of HA, BAP and a His6 epitope
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109424pET21d-myc-BirABacterial expression of biotin ligase (BirA) with an N-terminal myc epitope
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