Precise multimodal optical control of neural ensemble activity.
Mardinly AR, Oldenburg IA, Pegard NC, Sridharan S, Lyall EH, Chesnov K, Brohawn SG, Waller L, Adesnik H
Nat Neurosci. 2018 Apr 30. pii: 10.1038/s41593-018-0139-8. doi: 10.1038/s41593-018-0139-8. PubMed Article
Mardinly AR, Oldenburg IA, Pegard NC, Sridharan S, Lyall EH, Chesnov K, Brohawn SG, Waller L, Adesnik H
Nat Neurosci. 2018 Apr 30. pii: 10.1038/s41593-018-0139-8. doi: 10.1038/s41593-018-0139-8. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 108902 | pCAG-ChroME-mRuby2-ST | Cation channelrhodopsin ChroME fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites for optogenetic excitation |
| 108912 | pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3 | Cation channelrhodopsin ChroME targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector |
| 108960 | pCAG-H2B-mRuby3-IRES-eGtACR1-ST | Anion channelrhodopsin GtACR1 fused to ER export signal and targeted to the neuronal soma and proximal dendrites under the control of internal ribosome entry sequence |
| 109048 | pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST | Anion channelrhodopsin GtACR1 fused to ER export signal and targeted to the neuronal soma and proximal dendrites under the control of internal ribosome entry sequence in a viral vector |
| 109124 | pCAG-C1V1-TT-mRuby2-ST | Cation channelrhodopsin C1V1 fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites |
| 109125 | pCAG-ChR2-mRuby2-ST | Cation channelrhodopsin ChR2 fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites |
| 109126 | pCAG-ChETA-mRuby2-ST | Cation channelrhodopsin ChETA fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites |
| 109127 | pCAG-ChroME(F243Y)-mRuby2-ST | Modified cation channelrhodopsin ChroME fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites |
| 109128 | pCAG-ChroME(S273A)-mRuby2-ST | Modified cation channelrhodopsin ChroME fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites |
| 109129 | pCAG-Chronos(M140T)-mRuby2-ST | Modified cation channelrhodopsin Chronos fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites |
| 109130 | pCAG-CsChR-mRuby2-ST | Cation channelrhodopsin CsChR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites |
| 109131 | pCAG-CoChR-mRuby2-ST | Cation channelrhodopsin CoChR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites |
| 109132 | pCAG-eiC++-mRuby2-ST | Artificial anion channelrhodopsin iC++ fused to ER export signal and fluorophore mRuby2 and targeted to the neuronal soma and proximal dendrites |
| 109133 | pCAG-ePsuACR-mRuby2-ST | Anion channelrhodopsin PsuACR fused to ER export signal and fluorophore mRuby2 and targeted to the neuronal soma and proximal dendrites |
| 109134 | pCAG-eNpHR3.0-mRuby2-ST | Halorhodopsin fused to mRuby2 and targeted to the neuronal soma and proximal dendrites |
| 109135 | pCAG-eArch3.0-mRuby2-ST | Archaerhodopsin fused to mRuby2 and targeted to the neuronal soma and proximal dendrites |
| 109136 | pCAG-eGtACR1-mRuby2-ST | Anion channelrhodopsin GtACR1 fused to ER export signal, mRuby2 and targeted to the neuronal soma and proximal dendrites |