PurposeModified cation channelrhodopsin ChroME fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||109128||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5855
- Total vector size (bp) 7763
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Insert Size (bp)1908
- Promoter CAG
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer Custom
- 3′ sequencing primer WPRE_R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCAG-ChroME(S273A)-mRuby2-ST was a gift from Hillel Adesnik (Addgene plasmid # 109128 ; http://n2t.net/addgene:109128 ; RRID:Addgene_109128)
For your References section:Precise multimodal optical control of neural ensemble activity. Mardinly AR, Oldenburg IA, Pegard NC, Sridharan S, Lyall EH, Chesnov K, Brohawn SG, Waller L, Adesnik H. Nat Neurosci. 2018 Apr 30. pii: 10.1038/s41593-018-0139-8. doi: 10.1038/s41593-018-0139-8. 10.1038/s41593-018-0139-8 PubMed 29713079