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(Plasmid #115150)


Item Catalog # Description Quantity Price (USD)
Plasmid 115150 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 2739
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    Dual SH231 gRNAs
  • Alt name
  • gRNA/shRNA sequence
    gcctcccccatagtaccat, Gatgtgctcactgagtctga
  • Species
    Streptococcus pyogenes
  • Insert Size (bp)
  • Promoter U6, CBh
  • Tags / Fusion Proteins
    • 3xFLAG-SV40NLS (N terminal on backbone)
    • GFP (C terminal on insert)
    • Nucleoplasmin NLS (C terminal on backbone)

Cloning Information

Resource Information

Depositor Comments

Please visit for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUS2-SH231 was a gift from Raymond Monnat & Michael Phelps (Addgene plasmid # 115150 ; ; RRID:Addgene_115150)
  • For your References section:

    New human chromosomal safe harbor sites for genome engineering with CRISPR/Cas9, TAL effector and homing endonucleases. Pellenz S, Phelps MP, Tang W, Hovde BT, Sinit R, Fu W, Li H, Chen E, Monnat R. bioRxiv 396390 10.1101/396390