Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pUS2-SH231
(Plasmid #115150)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 115150 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    custom
  • Backbone size w/o insert (bp) 2739
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Dual SH231 gRNAs
  • Alt name
    Cas9-T2A-GFP
  • gRNA/shRNA sequence
    gcctcccccatagtaccat, Gatgtgctcactgagtctga
  • Species
    Streptococcus pyogenes
  • Insert Size (bp)
    6866
  • Promoter U6, CBh
  • Tags / Fusion Proteins
    • 3xFLAG-SV40NLS (N terminal on backbone)
    • GFP (C terminal on insert)
    • Nucleoplasmin NLS (C terminal on backbone)

Cloning Information

Resource Information

Depositor Comments

Please visit https://www.biorxiv.org/content/early/2018/08/20/396390 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUS2-SH231 was a gift from Raymond Monnat & Michael Phelps (Addgene plasmid # 115150 ; http://n2t.net/addgene:115150 ; RRID:Addgene_115150)
  • For your References section:

    New human chromosomal safe harbor sites for genome engineering with CRISPR/Cas9, TAL effector and homing endonucleases. Pellenz S, Phelps MP, Tang W, Hovde BT, Sinit R, Fu W, Li H, Chen E, Monnat R. bioRxiv 396390 10.1101/396390