|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||115150||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2739
Vector typeMammalian Expression, CRISPR
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameDual SH231 gRNAs
gRNA/shRNA sequencegcctcccccatagtaccat, Gatgtgctcactgagtctga
Insert Size (bp)6866
- Promoter U6, CBh
/ Fusion Proteins
- 3xFLAG-SV40NLS (N terminal on backbone)
- GFP (C terminal on insert)
- Nucleoplasmin NLS (C terminal on backbone)
- Cloning method Gibson Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
Please visit https://www.biorxiv.org/content/early/2018/08/20/396390 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pUS2-SH231 was a gift from Raymond Monnat & Michael Phelps (Addgene plasmid # 115150 ; http://n2t.net/addgene:115150 ; RRID:Addgene_115150)
For your References section:New human chromosomal safe harbor sites for genome engineering with CRISPR/Cas9, TAL effector and homing endonucleases. Pellenz S, Phelps MP, Tang W, Hovde BT, Sinit R, Fu W, Li H, Chen E, Monnat R. bioRxiv 396390 10.1101/396390