|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||115143||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerSystem Biosciences
- Backbone size w/o insert (bp) 6087
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Alt nameBxb1 attP
Alt nameC31 attP
Insert Size (bp)1876
- Promoter EF1a
- Cloning method Gibson Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Please visit https://www.biorxiv.org/content/early/2018/08/20/396390 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSH231-EF1-Puro was a gift from Raymond Monnat & Michael Phelps (Addgene plasmid # 115143 ; http://n2t.net/addgene:115143 ; RRID:Addgene_115143)
For your References section:New human chromosomal safe harbor sites for genome engineering with CRISPR/Cas9, TAL effector and homing endonucleases. Pellenz S, Phelps MP, Tang W, Hovde BT, Sinit R, Fu W, Li H, Chen E, Monnat R. bioRxiv 396390 10.1101/396390