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pSH231-EF1-BLST-dCas9-VPR
(Plasmid #115148)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 115148 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 115148-D50 50 μg of DNA in Tris buffer $495
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DNA 115148-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    Piggybac
  • Backbone manufacturer
    System Biosciences
  • Backbone size w/o insert (bp) 6087
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BlastR-P2A-dCas9-VPR
  • Alt name
    Bxb1 attP
  • Alt name
    C31 attP
  • Species
    Synthetic
  • Insert Size (bp)
    7766
  • Promoter EF1a
  • Tags / Fusion Proteins
    • Nucleoplasmin NLS (N terminal on backbone)
    • SV40NLS (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/early/2018/08/20/396390 for bioRxiv preprint.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSH231-EF1-BLST-dCas9-VPR was a gift from Raymond Monnat & Michael Phelps (Addgene plasmid # 115148 ; http://n2t.net/addgene:115148 ; RRID:Addgene_115148)

  • For your References section:

    New Human Chromosomal Sites with "Safe Harbor" Potential for Targeted Transgene Insertion. Pellenz S, Phelps M, Tang W, Hovde BT, Sinit RB, Fu W, Li H, Chen E, Monnat RJ Jr. Hum Gene Ther. 2019 Jul;30(7):814-828. doi: 10.1089/hum.2018.169. Epub 2019 Mar 28. 10.1089/hum.2018.169 PubMed 30793977
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