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PurposesfGFP expressed from hypoxia-inducible YB_TATA promoter and dsRed expressed from consitutive CMV promoter
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 117399 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepcDNA3.1(+)
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSuperFolder GFP
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Alt namesfGFP
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byThe sfGFP gene was PCRed from a plasmid received from Dr. Pamela Silver's laboratory.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
4xHRE_YB TATA-sfGFP-CMV_dsRed was a gift from Yvonne Chen (Addgene plasmid # 117399 ; http://n2t.net/addgene:117399 ; RRID:Addgene_117399) -
For your References section:
Quantitative Analyses of Core Promoters Enable Precise Engineering of Regulated Gene Expression in Mammalian Cells. Ede C, Chen X, Lin MY, Chen YY. ACS Synth Biol. 2016 May 20;5(5):395-404. doi: 10.1021/acssynbio.5b00266. Epub 2016 Mar 1. 10.1021/acssynbio.5b00266 PubMed 26883397
