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Single-cell RNA-seq reveals novel regulators of human embryonic stem cell differentiation to definitive endoderm.
Chu LF, Leng N, Zhang J, Hou Z, Mamott D, Vereide DT, Choi J, Kendziorski C, Stewart R, Thomson JA
Genome Biol. 2016 Aug 17;17(1):173. doi: 10.1186/s13059-016-1033-x.
PubMed

Plasmids from Article

ID Plasmid Purpose  
83344T-2A-EGFP-PGK-PuroDonor vector to knocin EGFP into human Brachyury(T) locus
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83346T-STOP sgRNA-1The sgRNA at the stop codon of human Brachyury(T) locus
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