162,996 results

1. pUAS:Cas9T2ACre;U6:sgRNA1;U6:sgRNA2

   Plasmid

   #74010

   Purpose

   Tissue-specific knock-out in zebrafish, Cas9 and Cre driven by a UAS promoter

   Depositor

   Filippo Del Bene

   Article

   Di Donato et al Genome Res. 2016 Mar 8.

   Inserts

   5xUAS
   Cas9
   Cre
   U6 promoter
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. 19 pCOLI_A_SUMO

   Plasmid

   #160257

   Purpose

   N-term 6xHis SUMO 3C cleavable Amp resistant bacterial expression vector

   Depositor

   John Weir

   Article

   Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

   Type

   Empty backbone

   Use

   Tags

   6xHis and SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLJC2-Blast-SHMT2-3xFLAG

   Plasmid

   #233891

   Purpose

   lentiviral overexpression vector for SHMT2

   Depositor

   Jason Cantor

   Article

   Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

   Insert

   SHMT2 (SHMT2 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCEP4-H031gp160

   Plasmid

   #123272

   Purpose

   Mammalian expression plasmid for Env from the H031 HIV-1 isolate

   Depositor

   Erik Procko

   Article

   Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

   Insert

   HIV-1 (H031) Env
   

   Use

   Tags

   CD5 leader peptide

   Expression

   Mammalian

   Mutation

   Codon-optimized synthetic gene

   Promoter

   CMV

   Available since

   April 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSpCTRE-CD4

   Plasmid

   #114010

   Purpose

   Dox-inducible Cas9 expression with a CD4 selection marker

   Depositor

   Charles Rudin

   Article

   Hulton et al Nat Cancer. 2020 Mar;1(3):359-369. doi:

   Insert

   SpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. T4 td upstream intron-PABP

   Plasmid

   #189972

   Purpose

   Part 1 plasmid to introduce the T4 td upstream intron and PABP spacer into circRNAs

   Depositor

   Howard Chang

   Article

   Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

   Insert

   T4 td upstream intron-PABP
   

   Use

   Cloning

   Tags

   Expression

   Mutation

   Promoter

   N/A

   Available since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. T-2A-EGFP-PGK-Puro

   Plasmid

   #83344

   Purpose

   Donor vector to knocin EGFP into human Brachyury(T) locus

   Depositor

   James Thomson

   Article

   Chu et al Genome Biol. 2016 Aug 17;17(1):173. doi:

   Insert

   Human Brachyury knockin homology arms (TBXT Human)
   

   Use

   Tags

   2A-EGFP

   Expression

   Mutation

   Promoter

   Available since

   Feb. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. IL1RN gRNA3

   Plasmid

   #113132

   Purpose

   Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   IL1RN guideRNA 3 (SpCas9 scaffold), co-expressed GFP (transfection marker)
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRVL-4

   Plasmid

   #104582

   Purpose

   Contains human kappa CL domain, for cloning of antibody VL domains using SapI restriction enzyme.

   Depositor

   Daniel Christ

   Article

   Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi

   Insert

   Human CL kappa
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEA186

    Plasmid

    #187874

    Purpose

    Empty pVS1-based INTEGRATE vector with mCherry cargo

    Depositor

    Kan Wang

    Article

    Aliu et al Plant Biotechnol J. 2022 Jun 11. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV flag ERR alpha

    Plasmid

    #10975

    Depositor

    Toren Finkel

    Article

    Ichida et al J Biol Chem. 2002 Dec 27. 277(52):50991-

    Insert

    ERR alpha (ESRRA Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 23, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCR8 JARID2

    Plasmid

    #114443

    Purpose

    Entry vector for gateway cloning containing human JARID2 coding sequence

    Depositor

    Adrian Bracken

    Article

    Conway et al Mol Cell. 2018 May 3;70(3):408-421.e8. d

    Insert

    JARID2 (JARID2 Human)
    

    Use

    Gateway entry cloning

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. qTAG-N-Blast-ultraID

    Plasmid

    #207682

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-ultraID cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pHR-CMV_TetR-HA-NLS-P2A-BSD-Myc

    Plasmid

    #113899

    Purpose

    Mammalian constitutive expression of HA-tagged Tetracyclin repressor protein and Myc-tagged blasticidin deaminase

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Insert

    TetR-HA-NLS-P2A-BSD-Myc
    

    Use

    Lentiviral

    Tags

    HA-Myc

    Expression

    Mutation

    Promoter

    CMV-MIE

    Available since

    Aug. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-NP-V5-B/BRISBANE/60/2008

    Plasmid

    #187923

    Purpose

    Expresses C terminal V5 tagged nucleoprotein of Influenza B/Brisbane/60/2008 virus in mammalian cells

    Depositor

    Arindam Mondal

    Article

    Kedia et al Front Microbiol. 2022 May 25;13:868367.

    Insert

    Influenza B Nucleoprotein
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX-6P-1_WT RAD23B

    Plasmid

    #201457

    Purpose

    Expresses human RAD23B with an N-terminal GST tag in E. coli

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog B (RAD23B Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Coding sequence has been optimised for expression…

    Promoter

    tac promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR223_PER1_WT

    Plasmid

    #82152

    Purpose

    Gateway Donor vector containing PER1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, William Hahn, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    PER1 (PER1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVX-TREtight-Ngn2:2A:Ascl1-PGK-Puro (XTP-N2A)

    Plasmid

    #84777

    Purpose

    Second generation lenti expressing Mash1 and Neurogenin for iN transdifferentation to neurons

    Depositor

    Fred Gage

    Article

    Mertens et al Cell Stem Cell. 2015 Oct 6. pii: S1934-5

    Insert

    Ngn2 and Mash1 with a T2A (NEUROG2, ASCL1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    pTIGHT

    Available since

    Nov. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV.hSynapsin.(cyto).iATPSnFR2.A95A.A119L.HaloTag

    Plasmid

    #209665

    Purpose

    Expression of iATPSnFR2, low affinity

    Depositor

    Tim Brown, HHMI-JRC Tool Translation Team

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    iATPSnFR2.A95A.A119L.HaloTag
    

    Use

    AAV

    Tags

    Expression

    Mutation

    A95A.A119L

    Promoter

    hSyn

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCL-CTIG

    Plasmid

    #14901

    Purpose

    3rd generation lentiviral vector expressing tTA-VP16 and EGFP.

    Depositor

    Inder Verma

    Article

    Kafri et al Mol Ther. 2000 Jun . 1(6):516-21.

    Insert

    CMV-tTA-GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 11, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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