159,258 results

1. pTEF2

   Plasmid

   #43971

   Depositor

   Hal Alper

   Article

   Crook et al Nucleic Acids Res. 2011 Aug;39(14):e92.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   TEF

   Available since

   April 9, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. BIC-Gag-CAS9

   Plasmid

   #119942

   Purpose

   encodes a GAG (F-MLV)-CAS9(sp) fusion. Allows the production of GAG-CAS9 Virus like particles from producer cells in association with over expressed gRNA(s) and appropriate envelopes.

   Depositor

   Emiliano Ricci, Philippe Mangeot, Théophile Ohlmann

   Article

   Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

   Insert

   GAG FMLV fused to spCAS9
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   hCMV

   Available since

   April 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBAD-LSSmOrange

   Plasmid

   #37129

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al J Am Chem Soc. 2012 Apr 24.

   Insert

   LSSmOrange
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

   Promoter

   araB

   Available since

   June 5, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKanB alpha

   Plasmid

   #223524

   Purpose

   Used for expression of secreted recombinant protein in Pichia pastoris; with G418 resistance gene as selectable marker

   Depositor

   Geoff Lin-Cereghino

   Article

   Lin-Cereghino et al Yeast. 2008 Apr;25(4):293-9. doi: 10.100

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   AOX1

   Available since

   Sept. 11, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. lentiTet-LDLR-mCherry BlastR

   Plasmid

   #186739

   Purpose

   Dox inducible LDLR coding sequence expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene

   Depositor

   Richard Sherwood

   Article

   Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

   Insert

   LDLR (LDLR Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSyn-ConVERGD-(HA)hM4Di-W3SL

   Plasmid

   #218759

   Purpose

   Provides AND intersectional (Cre+Flp) expression of HA-hM4Di

   Depositor

   Lindsay Schwarz

   Article

   Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

   Insert

   hM4Di
   

   Use

   AAV

   Tags

   HA

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   July 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBAD-luxOFP

   Plasmid

   #229945

   Purpose

   Bacterial expression of a de novo designed, FRET-based neoLux1.2-CyOFP1 fusion bioluminescent probe (max. Em=588)

   Depositor

   Andy Yeh

   Article

   Chen et al Chem. 2024 Nov 12. doi: 10.1016/j.chempr

   Insert

   luxOFP
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. Sec61b-HA-mNG21-10

   Plasmid

   #141213

   Purpose

   Membrane Tethered Split Fluorescent Reporter for ER Retragrade

   Depositor

   Wayne Lencer

   Article

   Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

   Insert

   Sec61b-HA-mNG21-10
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR221_HUWE1_isoform1

   Plasmid

   #190464

   Purpose

   Entry clone

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

   Insert

   HUWE1 (HUWE1 Human)
   

   Use

   Entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-DIO-SYP-HRP

    Plasmid

    #117186

    Purpose

    Plasmid name in publication: pAAV-DIO-SV-HRP. Peroxidase reporter for multiplexed electron microscopy labeling

    Depositor

    David Ginty

    Article

    Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

    Insert

    SYP-HRP
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRetroX-PTuner DD-linker-M.EcoGII

    Plasmid

    #122082

    Purpose

    Express M.EcoGII with detabilization domain - inducible

    Depositor

    Laure Crabbe

    Article

    Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

    Insert

    DD-linker-M.EcoGII
    

    Use

    Retroviral

    Tags

    V5 tag on Nter of M.EcoGII

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. mApple-PCNA-19-NLS-4

    Plasmid

    #54937

    Purpose

    Localization: Nucleus, Excitation: 568, Emission: 592

    Depositor

    Michael Davidson

    Insert

    PCNA-19 NLS (PCNA Human)
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3.1-hRXRα

    Plasmid

    #135910

    Purpose

    Human Retinoid X Receptor-alpha in pcDNA3.1(+) plasmid expression vector using SV40 Promoter

    Depositor

    Catharine Ross

    Article

    Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

    Insert

    Human retinoid X receptor- alpha (RXRA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. miniCMV-(mNeonGreen)4-tDeg

    Plasmid

    #129402

    Purpose

    (mNeonGreen)4-tDeg fluorogenic protein

    Depositor

    Samie Jaffrey

    Article

    Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

    Insert

    (mNeonGreen)-tDeg
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    miniCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)

    Available since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRK7-FLAG-TSC1

    Plasmid

    #8995

    Depositor

    John Blenis

    Article

    Tee et al Proc Natl Acad Sci U S A. 2002 Oct 15. 9

    Insert

    TSC1 (TSC1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 11, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-CMV-FLEX-SaCas9-U6-sgDrd1

    Plasmid

    #159917

    Purpose

    Mutagenesis of Drd1

    Depositor

    Larry Zweifel

    Insert

    Drd1 (Drd1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFRT_TO_FlagHA_G3BP1

    Plasmid

    #127106

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    G3BP1 (G3BP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFA6a-kanMX6

    Plasmid

    #39296

    Depositor

    John Pringle, Jurg Bahler

    Article

    Bahler et al Yeast. 1998 Jul;14(10):943-51.

    Insert

    KanR
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. p3xFLAG-CMV14-GINIP

    Plasmid

    #204845

    Purpose

    Express 3x FLAG tagged-GINIP in mammalian cells

    Depositor

    Mikel Garcia-Marcos

    Article

    Park et al Mol Cell. 2023 Jul 20;83(14):2540-2558.e

    Insert

    PHF24 (KIAA1045 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMJ179

    Plasmid

    #85996

    Purpose

    One-guide Perturb-seq vector backbone; modified mouse U6 promoter; constant region 2

    Depositor

    Jonathan Weissman

    Article

    Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

    Inserts

    EGFP-NT2_cr2
    puro-T2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    modified mouse U6

    Available since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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