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Flag-Jun4A-Myc
(Plasmid #47444)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 47444 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCMV-Tag2A
  • Backbone size w/o insert (bp) 4300
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Jun 4A mutant
  • Alt name
    Junc
  • Alt name
    c-Jun
  • Alt name
    AP-1
  • Species
    M. musculus (mouse)
  • Mutation
    four N-terminal residues phosphorylated by JNK, serines 63 and 73, and threonines 91 and 93, are mutated into alanines
  • Entrez Gene
    Jun (a.k.a. AP-1, Junc, c-ju, c-jun)
  • Promoter CMV
  • Tags / Fusion Proteins
    • Flag (N terminal on backbone)
    • myc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (destroyed during cloning)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Flag-Jun4A-Myc was a gift from Axel Behrens (Addgene plasmid # 47444 ; http://n2t.net/addgene:47444 ; RRID:Addgene_47444)
  • For your References section:

    c-Jun N-terminal phosphorylation antagonises recruitment of the Mbd3/NuRD repressor complex. Aguilera C, Nakagawa K, Sancho R, Chakraborty A, Hendrich B, Behrens A. Nature. 2011 Jan 13;469(7329):231-5. doi: 10.1038/nature09607. Epub 2011 Jan 2. 10.1038/nature09607 PubMed 21196933