Purpose(Empty Backbone) Lentiviral cDNA/shRNA gateway destination vectors
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||32685||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepLKO.1 puro
- Backbone size (bp) 7032
Modifications to backboneGateway destination cassette
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
Growth Strain(s)ccdB Survival
Copy numberLow Copy
- Cloning method Gateway Cloning
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The full plasmid sequence was digitally assembled and may contain minor discrepancies, which should not effect the function of the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLKO.DEST.hygro was a gift from Ming-Sound Tsao (Addgene plasmid # 32685 ; http://n2t.net/addgene:32685 ; RRID:Addgene_32685)
For your References section:Modified gateway system for double shRNA expression and Cre/lox based gene expression. Radulovich N, Leung L, Tsao MS. BMC Biotechnol. 2011 Mar 22;11:24. 10.1186/1472-6750-11-24 PubMed 21418658